Mahidol – Evident Bioimaging Center – Central Instrument Facility

Inverted Confocal Laser Scanning Microscope (CLSM FV4000)

Brand : Olympus
Model : FV4000
Location : B200 Room, 2nd Floor, B Building, Phyathai Campus
Custodian : Wattapong Pinyo

Service Fee :

SC staff	Mahidol	Government	Private
1,500 Baht/hour	2,000 Baht/hour	2,500 Baht/hour	3,500 Baht/hour

Description and Specification : Confocal laser scanning system equipped with research motorized inverted microscope, with advanced imaging technology enables the acquisition of higher precision images, empowering researchers with more reliable data from their samples. With SilVIR™ detector at the core of the system, you can achieve much lower noise, higher sensitivity, and improved photon resolving capabilities. The system also can support the live cell imaging with stage top incubator system.

4 Filter cubes installed :
• FUNA Excitation 360-370 Emission 420-460
• FBNA Excitation 470-495 Emission 510-550
• FGWA Excitation 530-550 Emission 575-625
• FYW Excitation 545-585 Emission >600

Objective lens:
• Universal Plan Apochromat 1.25X NA 0.04
• Universal Plan Extended Apochromat 10X NA 0.40
• Universal Plan Extended Apochromat 20X NA 0.80
• Universal Plan Extended Apochromat 40X NA 0.95
• Universal Plan Extended Apochromat 60X NA 1.42 – Oil Immersion
• Universal Plan Extended Apochromat 100X NA 1.45 – Oil Immersion

Visible Laser : 405 nm, 488 nm, 561 nm, 640 nm
NIR Laser : 785 nm

Scanner : Hybrid Scanner (Galvanometer & Resonant Scanner)
Detector (Camera) :
• 2CH Broadband SilVIR Detector
• 2CH Red-Shift SilVIR Detector
• 1CH Transmitted Detector

Observation Methods :
• Bright Field
• DIC
• Wide Field Fluorescence

Sample Type:

• glass slide with cover slip

• well plate (only 10x len)

APX100 (Benchtop Fluorescence Microscope)

Brand : Olympus
Model : APX100 (Benchtop Fluorescence Microscope)
Location : B200 Room, 2nd Floor, B Building, Phyathai Campus
Custodian : Wattapong Pinyo

Service Fee :

SC staff	Mahidol	Government	Private
500 Baht/hour	750 Baht/hour	1,000 Baht/hour	1,500 Baht/hour

Description and Specification :

The APX100 is an integrated digital imaging platform offering brightfield, phase contrast, gradient contrast, and fluorescence imaging. Designed for ease of use, the system automates sample loading, focusing, and acquisition to deliver consistent, high-quality images. With its built-in stage-top incubator, the APX100 also supports stable live-cell imaging for time-lapse experiments, making it suitable for a wide range of applications within core facilities.

Applicable observation method :
• Brightfield
• Gradient contrast
• Phase contrast
• Fluorescence

3 Filter cubes installed :
• FUNA : Excitation 360-370, Emission 420-460 DAPI
• FBNA : Excitation 470-495, Emission 510-550 FITC, GFP, Alexa Fluor 488
• FGW : Excitation 530-550, Emission 575IF TRITC, Nile Red

Objective lens :
• 4X: UPLFLN4XPH NA: 0.13
• 10X: UPLXAPO10X NA: 0.4
• 20X: LUCPLFLN20XPH NA: 0.45
• 40X: LUCPLFLN40XPH with Correction Collar NA: 0.6
• 60X (oil): UPLXAPO60XO NA: 1.42

Stage top incubator : Tokai Hit Incubator (Stage heater 10-50 Celsius and CO2 concentration range 5%-20%)

Detector (Camera) :
• DP23
• Hamamatsu ORCA-Fusion

Sample Type with Stage top incubator :
• Slide glass 1×3″
• Ø35 mm dish
• Ø60 mm dish
• Chamber slide
• Well plate

Sample Type without Stage top incubator :
• Slide glass 1×3″
• Ø35mm dish
• Well plate (glass bottom, plastic bottom/ 6 – 96wells/type with flatbottom holes)
• Flask: T25
• Dish: ø50mm, ø60mm
• Hemocytometer

Abbelight SAFe 360 Inverted single-molecule localisation microscope (SMLM)

Brand : Abbelight
Model : SAFe 360
Location : B200 Room, 2nd Floor, B Building, Phyathai Campus
Custodian : Wattapong Pinyo

Service Fee :

SC staff	Mahidol	Government	Private
550 Baht/hour	650 Baht/hour	750 Baht/hour	850 Baht/hour

Abbelight Buffer (Baht/Sample) :

SC staff	Mahidol	Government	Private
1,000 Baht/Sample	1,000 Baht/Sample	1,000 Baht/Sample	1,000 Baht/Sample

Description and Specification :

Single Molecule Localization Microscopy (SMLM)

– Super-resolution Fluorescence imaging system @20 nm
– Nanoscale visualization of cellular structures in 3D with localization precision @15x15x15 nm
– Compatible with SMLM methods such as STORM, PALM, DNA-PAINT
– ASTER Technology for wider Field Of View @150×150 micron
– Illumination modes: EPI (Transmitted) / HiLo (1-3 micron from surface) / TIRF (100-200nm)
– Analysis software supported Custer Analysis / Single Particle Tracking / Colocalization / Spectral Demixing

Laser light source : 405, 488, 561, 640 nm

Objective lens : UPLAPO 100X OHR

Detector (Camera) : Hamamatsu / ORCA-Fusion 2 sets

Sample Type : Fixed Sample slide / Live cell in ibidi micro-slide or bottom glass slide

VS200 Multi tray load (Research Slide Scanner)

Brand : Olympus
Model : VS200 Multi tray load (Research Slide Scanner)
Location : B200 Room, 2nd Floor, B Building, Phyathai Campus
Custodian : Wattapong Pinyo

Service Fee :

SC staff	Mahidol	Government	Private
100 Baht/slide	200 Baht/slide	300 Baht/slide	400 Baht/slide

Description and Specification :

Research slide scanner support Bright field and Fluorescence (DAPI, FITC, TRITC) observation method. Use with slide glass in maximum load 60 slides. Scan with autofocusing and automatic stitching. Selectable for normal plane (1 plane), Z stack (Maximum 41 planes) and EFI in both of bright field and fluorescence.

3 Filter cubes installed :
• U-FUW : Excitation 340-390, Emission 420IF : DAPI
• U-FBW : Excitation 460-495, Emission 510IF : FITC
• U-FGW : Excitation 530-550, Emission 575IF : TRITC

Objective lens :
• 2X dry : PLN – NA 0.06
• 4X dry : UPLFLN – NA 0.13
• 10X dry : UPLFLN – NA 0.3
• 20X dry : UPLXAPO – NA 0.8
• 40X dry : UPLFLN – NA 0.74
• 100X oil : UPLXAPO O – NA 1.42

Detector (Camera) :
• BF : Color camera model VS-264C
• FL : Monochrome camera model VS-304M

Sample Type : Glass slide with cover slip (Size 1″x3″)

Extra information Main component :
• VS200 Base Unit = 1
• Loader = 1
• LED Fluorescence Light Source = U-LGPS wavelength range 360 to 665 nm
• Tray = 10 pcs size 1″ x 3″

Techniques : Bright field and Fluorescence (DAPI, FITC, TRITC)

Inverted Confocal Laser Scanning Microscope (CLSM FV1000)

Brand : Olympus
Model : FV1000
Location : B200 Room, 2nd Floor, B Building, Phyathai Campus
Custodian : Wattapong Pinyo

Service Fee :

SC staff	Mahidol	Government	Private
1,000 Baht/hour	1,700 Baht/hour	2,200 Baht/hour	3,300 Baht/hour

Description and Specification :

FV1000 LASER

Excitation	Suitable Dyes
405nm	Alexa 405, DAPI, Hoechst
458nm	CFP
488nm	Alexa 488, FITC, GFP, Cy2
515nm	Alexa 514, YFP
543nm	Alexa 546, Alexa 568, TRITC, Cy3, DiI, Propidium Iodide, Texas red , mCherry
633nm	Alexa 633, Alexa 647, Cy5

Epifluorescence

Fluorescence can be observed on through IX-81 using the mercury lamp and filter cubes. We currently have 3 filter cubes installed.

• DAPI (Hoechst): ex 335-375, em 440-500
• FITC (EGFP/Alexa 488): ex 465-495, em 515-555
• TRITC (Alexa 546, Alexa 568, Alexa 633, Topro-3): ex 515-550, em 600-640

Objective lens :

• 10x air – UPlanSApo – NA 0.40
• 20x air – UPlanSApo – NA 0.75
• 40x air – UPlanSApo – NA 0.90 – with correction collar
• 60x oil – PlanSApo N – NA 1.42

Detector : Photomultiplier tube (PMT)

Sample Type:

• glass slide with cover slip

• well plate (only 10x len)

Fluorescence Microscope (BX53)

Brand : Olympus
Model : BX53
Location : B200 Room, 2nd Floor, B Building, Phyathai Campus
Custodian : Wattapong Pinyo

Service Fee :

SC staff	Mahidol	Government	Private
150 Baht/hour	200 Baht/hour	250 Baht/hour	350 Baht/hour

Description and Specification :

4 filter cubes installed.

Filter No.	Unit Name	Excitation Filter	Emission Filter	Dichromatic Mirror
1	U-FBNA Blue Excitations	470 – 495	510 – 550	505
2	U-FBW Blue Excitations	460 – 495	510IF	505
3	U-FGW Green Excitations	530 – 550	575IF	570
4	U-FUW Ultraviolet Excitations	340 – 390	420IF	410

Objective lens :

• 2x air – PlanApo N – NA 0.08
• 4x air – UPlanFL N – NA 0.13
• 10x air – UPlanFL N – NA 0.30
• 20x air – UPlanFL N – NA 0.50
• 40x air – UPlanFL N – NA 0.75
• 60x oil – PlanApo N – NA 1.42
• 100x oil – UPlanFL N – NA 1.30

Detector : DP74

Sample Type:

• glass slide with cover slip