Our expertise includes isolation of bacteria from biological samples (e.g. food, soil, wastewater, biofilm). Pure culture of each isolate can be identified to species level using 16s rDNA sequencing with necessary biochemical tests (depending on the species of interest). Number of viable cells and spores in the sample can be quantified using a spread plate technique. In case that the microbial populations (microbiome) is of interest, a culture-independent analysis using Next-generation sequencing (NGS) can be employed. This technique allows for discovering of microbes that are ‘non-culturable’ or may be in relatively low abundance and therefore ‘not detectable’ using traditional methods.
- Isolation of bacteria from biological sample (12,000 THB/sample)
- Identification and characterization of bacterial isolate using 16s rDNA sequencing and biochemical tests (2,100 THB/isolate)
- Quantification of bacterial viable cell and/or endospore in biological sample (600 THB/sample)
- Evaluation of bacterial community (microbiome) in biological sample using Next Generation Sequencing (NGS) (18,000 THB/sample
- Wongbunmak A, Khiawjan S, Suphantharika M, Pongtharangkul T. BTEX- and naphthalene-degrading bacterium Microbacterium esteraromaticum strain SBS1-7 isolated from estuarine sediment. Journal of Hazardous Materials 2017;339:82-90.
- Khiawjan S, Suphantharika M, Pongtharangkul T. 2014. Isolation of BTEX degrading bacteria from petroleum contaminated seabed sludges. Proceedings of the 2nd ASEAN Plus Three Graduate Research Congress (2nd AGRC), February 5-7. Bangkok, Thailand.
- Leiim J, Suphantharika M, Pongtharangkul T. 2015. Isolation and characterization of xylenes degrading-bacteria, Pseudomonas citronellolis PC1. Proceedings of The 27th Annual Meeting of the Thai Society for Biotechnology and International Conference, November 18-19. Bangkok, Thailand.
- Prasitwuttisak, W, Sapsanyakorn, K, Ruanglek, V and Pongtharangkul, T. (2018) A study on effects of 1,3-butadiene exposure on bacterial community in activated sludge using next generation sequencing. The 186th The IIER International Conference, 26-27 August 2018, Taipei, Taiwan.