IMB Consulting

Medium optimization for maximum cell or target product (Bacteria)

A systematic optimization of cultivation medium and condition can be employed to maximize the production of cell, endospore as well as target protein (e.g. enzyme, recombinant protein). Appropriate adjustment and replacement of necessary medium component can significantly reduce the operation cost and therefore accommodate a scale-up production.

Our Publications:

• Jenjitwanich A., Vilai-ek L., Taypongsak P., Jetiyanon K and Pongtharangkul T. “Medium optimization for plant growth promoting rhizobacteria (PGPR) Bacillus cereus strain RS87”. The 30th Annual Meeting of the Thai Society for Biotechnology and International Conference. 22-23 November 2018, Bangkok, Thailand. (2018).
• Jenjitwanich A., Jetiyanon K and Pongtharangkul T. “Upscaling of RS87 endospore production process – from flask to semi-industrial scale cultivation”. The 31th Annual Meeting of the Thai Society for Biotechnology and International Conference. 10-12 November 2019, Bangkok, Thailand. (2019)

Enzymatic bioconversion (Whole-cell or free-enzyme) for bio-based chemical production

The use of a biocatalyst allows for a sustainable and environmental friendly production of various valuable chemicals. Appropriate selection of enzyme(s) and reaction condition(s) are critical for the successful process development. Many industrially important enzymes require expensive cofactor(s), therefore an efficient cofactor regeneration system is highly necessary. Our expertise includes the use of our efficient glucose dehydrogenase to regenerate cofactors for several oxygenase enzymes. The cofactor regeneration system can be applied in various platform including crude enzyme extract, purified enzyme, a whole-cell biocatalyst with a co-expression or a coupling whole-cell biocatalyst format.

Our Publications:

• Jenjitwanich A., Vilai-ek L., Taypongsak P., Jetiyanon K and Pongtharangkul T. “Medium optimization for plant growth promoting rhizobacteria (PGPR) Bacillus cereus strain RS87”. The 30th Annual Meeting of the Thai Society for Biotechnology and International Conference. 22-23 November 2018, Bangkok, Thailand. (2018).
• Jenjitwanich A., Jetiyanon K and Pongtharangkul T. “Upscaling of RS87 endospore production process – from flask to semi-industrial scale cultivation”. The 31th Annual Meeting of the Thai Society for Biotechnology and International Conference. 10-12 November 2019, Bangkok, Thailand. (2019)