Brand : Olympus
Model : FV1000
Location : B200 Room, 2nd Floor, B Building, Phyathai Campus
Custodian : Wattapong Pinyo
Service Fee :
SC staff | Mahidol | Government | Private |
1,000 | 1,700 | 2,200 | 3,300 |
Description and Specification :
FV1000 LASER
| Excitation | Suitable Dyes |
|---|---|
| 405nm | Alexa 405, DAPI, Hoechst |
| 458nm | CFP |
| 488nm | Alexa 488, FITC, GFP, Cy2 |
| 515nm | Alexa 514, YFP |
| 543nm | Alexa 546, Alexa 568, TRITC, Cy3, DiI, Propidium Iodide, Texas red , mCherry |
| 633nm | Alexa 633, Alexa 647, Cy5 |
Epifluorescence
Fluorescence can be observed on through IX-81 using the mercury lamp and filter cubes. We currently have 3 filter cubes installed.
• DAPI (Hoechst): ex 335-375, em 440-500
• FITC (EGFP/Alexa 488): ex 465-495, em 515-555
• TRITC (Alexa 546, Alexa 568, Alexa 633, Topro-3): ex 515-550, em 600-640
Objective lens :
• 10x air – UPlanSApo – NA 0.40
• 20x air – UPlanSApo – NA 0.75
• 40x air – UPlanSApo – NA 0.90 – with correction collar
• 60x oil – PlanSApo N – NA 1.42
Detector : Photomultiplier tube (PMT)
Sample Type:
• glass slide with cover slip
• well plate (only 10x len)
Brand : Olympus
Model : IX83
Location : B200 Room, 2nd Floor, B Building, Phyathai Campus
Custodian : Wattapong Pinyo
Service Fee :
SC staff | Mahidol | Government | Private |
100 | 250 | 350 | 500 |
Description and Specification :
A dedicated live-cell imaging microscope in multiple channels (both fluorescence and brightfield, including phase contrast and DIC with temperature, 5% CO2, humidity control) over long time periods (xyzt in multiple channels).
3 filter cubes installed.
• DAPI (Hoechst): ex 335-375, em 440-500
• FITC (EGFP/Alexa 488): ex 465-495, em 515-555
• TRITC (Alexa 546, Alexa 568, Alexa 633, Topro-3): ex 515-550, em 600-640
Objective lens :
• 4x air – UPlanFL N – NA 0.13
• 10x air – UPlanFL N – NA 0.30
• 20x air – LUCPlanFL N – NA 0.45 – with correction collar
• 20x air – UCPlanFL N – NA 0.70 – with correction collar
• 40x air – LUCPlanFL N – NA 0.60 – with correction collar
• 60x air – LUCPlanFL N – NA 0.70 – with correction collar
Detector : DP74
Sample Type:
• glass slide with cover slip
• well plate
Brand : Olympus
Model : BX53
Location : B200 Room, 2nd Floor, B Building, Phyathai Campus
Custodian : Wattapong Pinyo
Service Fee :
SC staff | Mahidol | Government | Private |
20 | 50 | 100 | 300 |
Description and Specification :
4 filter cubes installed.
Filter No. | Unit Name | Excitation Filter | Emission Filter | Dichromatic Mirror |
1 | U-FBNA Blue Excitations | 470 – 495 | 510 – 550 | 505 |
2 | U-FBW Blue Excitations | 460 – 495 | 510IF | 505 |
3 | U-FGW Green Excitations | 530 – 550 | 575IF | 570 |
4 | U-FUW Ultraviolet Excitations | 340 – 390 | 420IF | 410 |
Objective lens :
• 2x air – PlanApo N – NA 0.08
• 4x air – UPlanFL N – NA 0.13
• 10x air – UPlanFL N – NA 0.30
• 20x air – UPlanFL N – NA 0.50
• 40x air – UPlanFL N – NA 0.75
• 60x oil – PlanApo N – NA 1.42
• 100x oil – UPlanFL N – NA 1.30
Detector : DP74
Sample Type:
• glass slide with cover slip
Brand : Olympus
Model : MVX10
Location : B200 Room, 2nd Floor, B Building, Phyathai Campus
Custodian : Wattapong Pinyo
Service Fee :
SC staff | Mahidol | Government | Private |
100 | 250 | 350 | 500 |
Description and Specification :
2 filter cubes installed.
Filter No. | Unit Name | Excitation Filter | Emission Filter | Dichromatic Mirror |
1 | U-MGFPHQ | 460-480HQ | 495-540HQ | 485 |
2 | U-MRFPHQ | 535-555HQ | 570-625HQ | 565 |
Objective lens :
• 1x
• 2x
Seamless zoom observation from 6.3x to 126x
Detector : DP80
Sample Type:
• glass slide with cover slip
• well plate
Brand : Hitachi
Model : SU8010
Location : B200 Room, 2nd Floor, B Building, Phyathai Campus
Custodian : Wattapong Pinyo
Service Fee :
SC staff | Mahidol | Government | Private |
800 | 1,500 | 2,000 | 2,700 |
EDX (Baht/File) :
SC staff | Mahidol | Government | Private |
200 | 400 | 500 | 500 |
Description and Specification :
• Ultra high resolution imaging (Resolution 1.0nm/15kV)
• Beam deceleration function as standard (Resolution 1.3nm/1kV)
• SE-BSE signal mixing function (Super ExB)
Secondary Electron Image Resolution | 1.0 nm (Vacc 15 kV, WD=4 mm) 1.3 nm (landing voltage 1kV, WD=1.5 mm) |
Low mag mode | 20-2,000 × |
High mag mode | 100-800,000 × |
Electron gun | Cold cathode fi eld emission source |
Accelerating voltage | 0.5 kV to 30 kV(Standard mode) |
Landing voltage | 0.1 kV to 2.0 kV(Deceleration mode) |
Lens system | 3-stage electromagnetic lens reduction system |
Objective lens aperture | Objective aperture(Heating type), 4 openings selectable from outside of vacuum, fi nely adjustable |
Max. sample size | 100 mm dia. |
Brand : Hitachi
Model : E102
Location : B200 Room, 2nd Floor, B Building, Phyathai Campus
Custodian : Wattapong Pinyo
Service Fee :
SC staff | Mahidol | Government | Private |
300 | 450 | 600 | 900 |
Description and Specification :
Target : Platinum
Brand : Hitachi
Model : HCP-2
Location : B200 Room, 2nd Floor, B Building, Phyathai Campus
Custodian : Wattapong Pinyo
Service Fee :
SC staff | Mahidol | Government | Private |
200 | 300 | 400 | 600 |
Brand : Leica
Model : EM UC6
Location : B200 Room, 2nd Floor, B Building, Phyathai Campus
Custodian : Wattapong Pinyo
Service Fee :
SC staff | Mahidol | Government | Private |
50 | 100 | 200 | 300 |
Description and Specification :
