| |
|
CURRICULUM VITAE |
 |
| |
|
| Name : |
Assoc. Prof. Dr. Jeerus Sucharitakul |
| Affiliation : |
Department of Biochemistry,
Faculty of Dentistry,
Chulalongkorn University, Henri-Dunant
Road,
Bangkok, 10300, Thailand
Email:jsucharitakul@hotmail.com
|
| Education
: |
| 2005 |
Ph.D. Student (Biochemistry)
Mahidol University, Bangkok, Thailand. |
| 2001 |
M.Sc. (Biochemistry)
Mahidol University, Bangkok, Thailand. |
| 1997 |
D.D.S. Faculty of
Dentistry, Chulalongkorn University,
Bangkok,Thailand |
| Scholarship
and Honors: |
| 2008 |
Chulalongkorn
Best Research Award |
| 2000 |
Tab Nilanidhi’s
foundation award for outstanding
M.Sc. student |
| 2001 |
Fellowship from
Ministry of University affairs |
| Research
and workshop training experiences: |
| 2004 |
Research training
at Department of Biological Chemistry,
Medical School, University of
Michigan, Ann Arbor, MI, US |
| Field of
Interest: |
| |
Mechanistic Enzymology,
Enzyme Kinetics, Protein Purification |
| Publications: |
| |
1. Sucharitakul
J, Chaiyen P, Ballou D P, and
Massey V 2002. Probing the Mechanism
of 2-Methyl-3-Hydroxypyridine-5-Carboxylic
Acid Oxygenase by Using 8-Substituted-FAD
Analogs, in Flavins &
Flavoproteins, ( Eds
: Chapman, S., Perham, R., and
Scrutton, N.), Rudolf Weber, Berlin,
pp. 381-386. |
| |
2. Chaiyen P, Sucharitakul
J, Svasti J, Entsch B, Massey
V and Ballou DP. 2004. Use of
8-Substituted-FAD Analogues to
Investigate the Hydroxylation
Mechanism of the Flavoprotein
2-methyl-3-hydroxypyridine-5-carboxylic
acid oxygenase. Biochemistry
43, 3933-3943. |
| |
3. Thotsaporn K,
Sucharitakul J, Wongratana J,
Suadee C, Chaiyen P. 2004. Cloning
and expression of p-hydroxyphenylacetate
3-hydroxylase from Acinetobacter
baumanii: evidence of the divergence
of enzymes in the class of two-protein
component aromatic hydroxylase.
Biochem et Biophysica
Acta 1680, 60-66. |
| |
4. Oonanant W, Sucharitakul
J, Yuvaniyama J, Chaiyen P. 2005
Crytallization and preliminary
X-ray crystallographic analysis
of 2-methyl-3-hydroxypyridine-5-
carboxylic acid (MHPC) oxygenase
from Pseudomonas sp. MA-1. Acta
Cryst F, F61. |
| |
5. Sucharitakul
J, Chaiyen P, Entsch B, Ballou
DP. 2005 The reductase of p- hydroxyphenylacetate
3-hydroxylase from Acinetobacter
baumannii requires p- hydroxyphenylacetate
for effective catalysis. Biochemistry
44, 10434-10442. |
| |
6. Sucharitakul
J, Chaiyen P, Entsch B, Ballou
DP. 2006 Kinetic mechanisms of
the oxygenase from a two-component
enzyme, p-hydroxyphenylacetate
3-hydroxylase from Acinetobacter
baumannii. J Biol Chem.
281, 17044-17053.
|
| |
7. Kujawa M, Ebner
H, Leitner C, Hallberg BM, Prongjit
M, Sucharitakul J, Ludwig R, Rudsander
U, Peterbauer C, Chaiyen P, Haltrich
D, Divne C. 2006 Structural basis
for substrate binding and regioselective
oxidation of monosaccharides at
C3 by pyranose 2-oxidase. J
Biol Chem. 281,
35104-35115. |
| |
8. Sucharitakul
J, Phongsak T, Entsch B, Svasti
J, Chaiyen P, Ballou DP. 2007
Kinetics of a two-component p-hydroxyphenylacetate
hydroxylase explain how reduced
flavin is transferred from the
reductase to the oxygenase. Biochemistry
46, 8611-8623. |
| Research
presentations: |
| Oral
Presentation |
| |
1.
Sucharitakul J, Entsch B, Ballou
DP and Chaiyen P. 2004. Use of
pre-steady state kinetics to investigate
the reaction mechanism of the
two-component enzyme p-hydroxyphenylacetate
hydroxylase. The 17th FAOBMB Symposium/
2nd IUBMB Special Meeting/ 7th
A-IMBN Conference, Imprerial Queen’s
Park Hotel, Bangkok, Thailand.
|
| Poster |
| |
1. Sucharitakul
J and Chaiyen P. 1999. Coenzyme
engineering of 2-methyl-3- hydroxypyridine-5-carboxylic
acid oxygenase. 25 th Congress
on Science and Technology of Thailand
at Amarin Lagoon Hotel, Pitsanulok,
Thailand. |
| |
2. Sucharitakul
J and Chaiyen P. 2000. Chemical
modification of FAD (flavin adenine
dinucleotide) to elucidate the
mechanism of 2-methyl-3-hydroxypyridine-5-carboxylic
acid oxygenase (MHPCO). The 12
th Annual Meeting of the Thai
Society for Biotecnology at Felix
Hotel, Kanchanaburi, Thailand. |
| |
3. Sucharitakul
J and Chaiyen P. 2001. Probing
hydroxylation mechanim of 2-methyl-3-hydroxypyridine-5-carboxylic
acid (MHPC) oxygenase (MHPCO).
25 th Congress on Science and
Technology of Thailand at Lee
Gardens Plaza Hotel, Hat yai,
Songkla, Thailand . |
| |
4. Sucharitakul
J and Chaiyen P. 2003. Studies
of the reductase component of
p-hydroxyphenylacetate hydroxylase.
Joint Senior Research Scholar
Meeting at Royal River Hotel,
Thailand. |
| Skills: |
| |
- Gene
cloning and expression |
| |
- Protein
purification and enzyme assay |
| |
- Thermodynamic
and kinetic studies of Flavin
and Flavoproteins |
| |
- Steady
state kinetics |
| |
- Stopped-flow
techniques for investigating enzyme
reaction mechanisms |
| |
| |
