|
|
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Biography
:
|
|
| Name
: |
Prof. Pimchai Chaiyen,
Ph.D. |
| Main Position: |
Professor and Head |
| Address
: |
School of Biomolecular Science and
Engineering,
Vidyasirimedhi Inistitute of Science and Technology (VISTEC),
555 Moo 1 Payupnai,
Wangchan Valley, Rayong, Thailand 21210. |
| |
E-mail
: pimchai.chaiyen@vistec.ac.th
|
| |
Website
: www.vistec.ac.th/academic/home.php?school=BSE |
| |
Website
:
Chaiyen Research Group |
| |
|
| Adjunct Position: |
Professor |
| Address
: |
Department of Biochemistry /
Center for Excellence in Protein and Enzyme Technology |
| |
Faculty of Science, Mahidol University
272 Rama VI Road, Bangkok 10400, THAILAND |
| |
Website
: science.mahidol.ac.th/Chaiyen_P
|
|
Education:
|
| 1992 |
B.Sc. (First Class
Hons) Chemistry, Prince of Songkla
University,Thailand |
| 1997 |
Ph. D. Biological Chemistry,
University of Michigan, Ann Arbor,
U.S.A. |
|
|
|
Research
and Professional Experiences:
|
| 2017-present |
Professor and Head, School of Biomolecular Science and
Engineering, Vidyasirimedhi Institute of Science and Technology
(VISTEC) |
| 2009-2017 |
Professor, Dept of Biochemistry, Faculty of Science, Mahidol
University |
| 2005 |
Associate Prof, Dept of Biochemistry, Faculty of Science,
Mahidol University |
| 2001 |
Assistant Prof, Dept of Biochemistry, Faculty of Science,
Mahidol University |
| 1997 |
Lecturer, Dept of Biochemistry, Faculty of Science, Mahidol
University |
| 1994 |
Teaching assistant in Enzyme Kinetics for graduate students,
University of Michigan, Ann Arbor
|
| 1993-1996 |
Teaching assistant in Biochemistry Laboratory for
undergraduate and graduate students, University of Michigan,
Ann Arbor
|
| 2015-2017 |
Member
of Subcommittee for New Economy under the committees of
Thailand’s National Reform Steering Assembly |
| 2015-present |
Executive
board member, National Center for Genetic Engineering and
Biotechnology (BIOTEC), National Science and Technology
Development Agency (NSTDA) |
|
|
|
Administration and Leadership Positions:
|
| 2017-present |
Head, School of Biomolecular Science and Engineering,
Vidyasirimedhi Institute of Science and Technology (VISTEC) |
| 2016 |
Co-founder, Enzmart Biotech Co. |
| 2011-2015 |
Deputy Dean for Research, Faculty of Science, Mahidol
University
|
| 2009-2011 |
Chair, Dept of Biochemistry, Faculty of Science, Mahidol
University
|
| 2008-2009 |
Deputy Chair, Dept of Biochemistry, Faculty of Science,
Mahidol University
|
| 2018-present |
Acting Director, Frontier Research Center, Vidyasirimedhi Institute of Science and Technology (VISTEC)
|
|
|
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Awards
and Distinctions :
|
| 1985 |
Recipient of Distinguished Student
Award from Princess Sirindhorn |
| 1985-1997 |
Recipient of Scholarship from
Development and Promotion of Science
and Technology Talent Project |
| 1993 |
Recipient of Dr.Tap Nilaniti
Outstanding Graduate Award |
| 1995 |
Recipient of Chrisman Award
from Department of Biological
Chemistry, University of Michigan,
Ann Arbor. (for outstanding Ph.D.
candidate) |
| 1998 |
Recipient of Murphy Award from
Department of Biological Chemistry,
University of Michigan, Ann Arbor.
(for the outstanding publication
series) |
| 2000 |
Recipient of Federation of Asian
and Oceanic Biochemistry and Molecular
Biology (FAOBMB) travel fellowship |
| 2003 |
Recipient of the L'oreal-Unesco
Fellowship for Woman in Science
in Thailand |
| 2005 |
Young Scientist Award, Foundation
for the Promotion of Science and
Technology under the Patronage
of H. M. the King, Thailand. |
| 2008 |
Affiliate Fellow of TWAS (The
Academy of Sciences for the Developing
World) |
| 2009 |
BMB Award from Section of Biochemistry
and Molecular Biology, The Science
Society of Thailand under the
Patronage of His Majesty the King. |
| 2009 |
Faculty of Science Outstanding
Lecturer Award (First-Middle Level) |
| 2010 |
Exemplary Lecturer Award from
Mahidol University Faculty Senate |
| 2010 |
Taguchi Prize for Outstanding
Research Achievement in Biotechnology |
| 2010 |
TRF-CHE-Scopus Researcher Award
2010 |
| 2011 |
Outstanding Alumni Award 2011 from Prince of Songkla
University |
| 2012 |
2012 Outstanding Researcher Award (Chemical Sciences and
Pharmacy Section) from the National Research Council of Thailand
(NRCT) |
| 2013 |
Received the title "TRF Senior Research Scholar" and
Research Team Building Grant from The Thailand Research
Fund |
| 2014 |
Chair of the Organizing Committee, IUBMB 18th
International Symposium on Flavins and Flavoproteins |
| 2015 |
Speaker at TEDx Bangkok 2015 |
| 2015 |
Outstanding Scientist of Thailand 2015, Foundation for the
Promotion of Science and Technology under the Patronage of H. M.
the King, Thailand. |
| 2015 |
Outstanding Alumni Award from Royal Thai Government
Scholarship Alumni Association |
| 2016 |
BioTalk Plenary Awardee from Biotechnology and Biochemical
Engineering Society of Taiwan (BEST) |
| 2016 |
The project “bacterial luciferase as a gene reporter)
received a special award from Leave A Nest Co. to participate in
the Tech Planter Final Grand Prix in Tokyo, Japan (Sep 17, 2016)
|
| 2016 |
Received the title "TRF Senior Research Scholar" and Research
Team Building Grant from The Thailand Research Fund |
| 2017 |
Received Innovation Award for “Protein Markers”
from the National Research Council of Thailand (NRCT) |
| 2017 |
Received Research Excellence Award for “Serine
hydroxymethyltransferase as a malarial drug target” |
| 2017 |
Received First Place in Final Pitch Session “Leaders in
Innovation Fellowship” hosted by Royal Academy
of Engineering and Newton Fund, UK
|
| 2017 |
L'oreal-Unesco Woman in Science Crystal Award for the most
accomplished woman scientist in Thailand (Life Science) |
| 2019 |
Distinguished Alumni Lecture, Department of Biological
Chemistry,
University of Michigan, Ann Arbor, USA
|
| 2019 |
Outstanding Protein Scientist of Thailand |
| 2020 |
Honorable mention invention from NRCT "BioVis fermentation unit for high efficiency biogas and biofertilizer production" |
| 2020 |
Honorable mention invention from NRCT "Smart pesticide detection for food safety" |
| 2020 |
Research Excellence Award for "Pyranose 2-oxidase as an efficient biocatalyst for sugar conversion" from the National Research Council of Thailand (NRCT) |
|
|
|
Editorial Board Members :
|
| |
The Journal of Biological Chemistry (JBC) (from July 2012-present) |
| |
Archives of Biochemistry and Biophysics (from November 2012-present) |
| |
eLife (from June 2019-present) |
| |
|
|
Ad Hoc Journal Reviewer :
|
| |
ACS Catalysis
|
| |
Acta Crystallographica Section F |
| |
Applied Environmental Microbiology |
| |
Applied Microbiology and Biotechnology |
| |
Advanced Synthesis & Catalysis |
| |
Archives of Biochemistry and Biophysics |
| |
Biochemistry |
| |
Biochimie |
| |
Biochimica Biophysica Acta |
| |
Bioresource Technology |
| |
Biotechnology Advances |
| |
Biotechnology & Bioengineering |
| |
Biotechnology Journal |
| |
Chemistry & Biology |
| |
ChemCatChem |
| |
Chemical Science |
| |
FEBS Journal |
| |
FEMS Microbiology Letter |
| |
Journal of Agricultural and Food Chemistry |
| |
The Journal of Biological Chemistry (JBC) |
| |
Journal of Chemical Education |
| |
Journal of Molecular Catalysis B: Enzymatic |
| |
Journal of the American Chemical Society (JACS) |
| |
Journal of Photochemistry and Photobiology B: Biology |
| |
Microbial Cell Factories |
| |
Nature Chemical Biology |
| |
PNAS |
| |
Trends in Biochemical Science |
| |
|
|
Reviewer
of Funding Organizations :
|
| |
Austrian
Science Fund |
| |
National Science Foundation
(NSF, USA.)
|
| |
The
Thailand Research Fund (TRF) |
| |
National
Science and Technology Development
Agency (NSTDA) |
| |
Commission
on Higher Education (CHE),
Ministry of Education |
| |
Mahidol
University |
| |
|
|
Field
of Interest :
|
| |
Enzyme catalysis; Enzyme mechanism; Enzyme engineering
Biocatalysis; Biorefinery; Bioenergy
Biosensor; Bioreporter
Metabolic Engineering; Synthetic Biology
Flavins and Flavoproteins; Aldolase; Redox Enzymes;
pyridoxal-5-phosphate enzymes
|
|
|
|
Publications
:
|
|
Journal
Articles
|
| 1997 |
| 1. |
Chaiyen
P, Brissette P, Ballou
DP, Massey V. Reaction of
2-methyl-3-hydroxypyridine-5-carboxylic
acid (MHPC) oxygenase with N-methyl-5-hydroxynicotinic
acid: Studies on the mode of binding,
and protonation status of the
substrate. Biochemistry
1997;36(45):13856-64. |
| |
|
| 2. |
Chaiyen
P, Ballou DP, Massey
V. Gene cloning, sequence analysis,
and expression of 2-methyl-3-hydroxypyridine-5-carboxylic
acid oxygenase. Proceedings
of the National Academy of Sciences
of the United States of America
1997;94(14):7233-8. |
| |
|
| 3. |
Chaiyen
P, Brissette P, Ballou
DP, Massey V. Unusual mechanism
of oxygen atom transfer and product
rearrangement in the catalytic
reaction of 2-methyl-3-hydroxypyridine-5-carboxylic
acid oxygenase. Biochemistry
1997;36(26):8060-70. |
| |
|
| 4. |
Chaiyen
P, Brissette P, Ballou
DP, Massey V. Thermodynamics and
reduction kinetics properties
of 2-methyl-3- hydroxypyridine-5-carboxylic
acid oxygenase.
Biochemistry 1997;36(9):2612-21.
|
| |
|
| 2001 |
| 5. |
Chaiyen
P, Suadee C, Wilairat
P. A novel two-protein component
flavoprotein hydroxylase p-hydroxyphenylacetate
hydroxylase from Acinetobacter
baumannii. European
Journal of Biochemistry 2001;268(21):5550-61. |
| |
|
| 2004 |
| 6. |
Chaiyen
P, Sucharitakul J, Svasti
J, Entsch B, Massey V, Ballou
DP. Use of 8-Substituted-FAD Analogues
to Investigate the Hydroxylation
Mechanism of the Flavoprotein
2-Methyl-3-hydroxypyridine-5-carboxylic
Acid Oxygenase. Biochemistry
2004;43(13):3933-43. |
| |
|
| 7. |
Thotsaporn
K, Sucharitakul J, Wongratana
J, Suadee C, Chaiyen P.
Cloning and expression of p-hydroxyphenylacetate
3-hydroxylase from Acinetobacter
baumannii: Evidence of the
divergence of enzymes in the class
of two-protein component aromatic
hydroxylases. Biochimica
et Biophysica Acta - Gene Structure
and Expression 2004;1680(1):60-6. |
| |
|
| 2005 |
| 8. |
Hommalai
G, Chaiyen P,
Svasti J. Studies on the transglucosylation
reactions of cassava and Thai
rosewood b-glucosidases
using 2-deoxy-2-fluoro-glycosyl-enzyme
intermediates. Archives
of Biochemistry and Biophysics
2005;442(1):11-20. |
| |
|
| 9. |
Oonanant
W, Sucharitakul J, Yuvaniyama
J, Chaiyen P.
Crystallization and preliminary
X-ray crystallographic analysis
of 2-methyl-3-hydroxypyridine-5-carboxylic
acid (MHPC) oxygenase from Pseudomonas
sp. MA-1. Acta
Crystallographica Section F: Structural
Biology and Crystallization Communications
2005;61(3):312-4. |
| |
|
| 10. |
Sucharitakul
J, Chaiyen P,
Entsch B, Ballou DP. The reductase
of p-hydroxyphenylacetate 3-hydroxylase
from Acinetobacter baumannii
requires p-hydroxyphenylacetate
for effective catalysis. Biochemistry
2005;44(30):10434-42. |
| |
|
| 2006 |
| 11. |
Kujawa
M, Ebner H, Leitner C, Hallberg
BM, Prongjit M, Sucharitakul J,
Ludwig R, Rudsander U, Peterbauer
C, Chaiyen P,
Haltrich D, Divne C. Structural
basis for substrate binding and
regioselective oxidation of monosaccharides
at C3 by pyranose 2-oxidase. Journal
of Biological Chemistry 2006;281(46):35104-15. |
| |
|
| 12. |
Sucharitakul
J, Chaiyen P,
Entsch B, Ballou DP. Kinetic mechanisms
of the oxygenase from a two-component
enzyme, p-hydroxyphenylacetate
3-hydroxylase from Acinetobacter
baumannii. Journal
of Biological Chemistry 2006;281(25):17044-53. |
| |
|
| 2007 |
| 13. |
Alfieri
A, Fersini F, Ruangchan N, Prongjit
M, Chaiyen P,
Mattevi A. Structure of the monooxygenase
component of a two-component flavoprotein
monooxygenase. Proceedings
of the National Academy of Sciences
of the United States of America
2007;104(4):1177-82. |
| |
|
| 14. |
Suadee
C, Nijvipakul S, Svasti J, Entsch
B, Ballou DP, Chaiyen
P. Luciferase from Vibrio
campbellii is more thermostable
and binds reduced FMN better than
its homologues. Journal
of Biochemistry 2007;142(4):539-52. |
| |
|
| 15. |
Sucharitakul
J, Phongsak T, Entsch B, Svasti
J, Chaiyen P,
Ballou DP. Kinetics of a two-component
p-hydroxyphenylacetate
hydroxylase explain how reduced
flavin is transferred from the
reductase to the oxygenase. Biochemistry
2007;46(29):8611-23. |
| |
|
| 2008 |
| 16. |
Nijvipakul
S, Wongratana J, Suadee C, Entsch
B, Ballou DP, Chaiyen
P. LuxG is a functioning
flavin reductase for bacterial
luminescence. Journal
of Bacteriology 2008;190(5):1531-8. |
| |
|
| 17. |
Sucharitakul
J, Prongjit M, Haltrich D, Chaiyen
P. Detection of a C4a-Hydroperoxyflavin
Intermediate in the Reaction of
a Flavoprotein Oxidase.
Biochemistry 2008;47(33):8485-90.
|
| |
|
| 2009 |
| 18. |
Spadiut
O, Pisanelli I, Maischberger T,
Peterbauer C, Gorton L, Chaiyen
P, Haltrich D. Engineering
of pyyranose 2-oxidase: improvement
for biofuel cell and food applications
through semi-rational protein
design. Journal
of Biotechnology 2009;
139(3):250-7.
|
| |
|
| 19. |
Prongjit M, Sucharitakul
J, Wongnate T, Haltrich D, Chaiyen
P. Kinetic mechanism
of pyranose 2-oxidase from Trametes
multicolor.
Biochemistry 2009;48(19):4170-80.
|
| |
|
| 20. |
Chosrowjan H, Taniguchi
S, Mataga N, Phongsak T, Sucharitakul
J, Chaiyen P,
Tanaka F. Ultrafast Solvation
Dynamics of flavin mononucleotide
in the reductase component of
p-Hydroxyphenylacetate Hydroxylase.
Journal
of Physical Chemistry B 2009;113(25):8439-42. |
| |
|
| 21. |
Baron R, Riley
C, Chenprakhon P, Thotsaporn K,
Winter RT, Alfieri A, Forneris
F, van Berkel WJH, Chaiyen
P, Fraaije MW, Mattevi
A, McCammon JA. Multiple pathways
guide oxygen diffusion into flavoenzyme
active sites. Proceedings
of the National Academy of Sciences
of the United States of America
2009;106(25):10603-8. |
| |
[ Cited in
"Research Highlights"
Nature Chemistry, 2009] |
| |
|
| 22. |
Sopitthammakhun
K, Maenpuen S, Yuthavong Y, Leartsakulpanich
U, Chaiyen P.
Serine hydroxymethyltransferase
from Plasmodium vivax is different
in substrate specificity from
its homologues. FEBS
Journal 2009;276(15):4023-36.
|
| |
|
| 23. |
Maenpuen S, Sopitthammakhun
K, Yuthavong Y, Chaiyen
P, Leartsakulpanich U.
Characterization of Plasmodium
falciparum serine hydroxymethyltransferase-A
potential antimalarial target.
Molecular
and Biochemical Parasitology 2009;168(1):63-73.
|
| |
|
| 2010 |
| 24. |
Chaiyen
P. Flavoenzymes
catalyzing oxidative aromatic
ring-cleavage reactions. Arch
Biochem Biophys. 2010;493(1):62-70.
(Review) |
| |
|
| 25. |
Pitsawong W, Sucharitakul
J, Prongjit M, Tan TC, Spadiut
O, Haltrich D, Divne C, Chaiyen
P. A conserved active-site
threonine is important for both
sugar and flavin oxidations of
pyranose 2-oxidase. Journal
of Biological Chemistry 2010;285(13):9697-705. |
| |
|
| 26. |
Sucharitakul J,
Wongnate T, Chaiyen P.
Kinetic isotope effects on the
noncovalent flavin mutant protein
of pyranose 2-oxidase reveal insights
into the flavin reduction mechanism.
Biochemistry 2010;49(17):3753-65.
|
| |
|
| 27. |
Chenprakhon P,
Sucharitakul J, Panijpan B,
Chaiyen P. Measuring
binding affinity of protein. Ligand
interaction using spectrophotometry:
binding of neutral red to riboflavin-binding
protein. Journal
Chemical Education 2010;87(8):829-31. |
| |
|
| 28. |
Rujkorakarn R,
Nunthaboot N, Tanaka F,
Chaiyen P, Chosrowjan
H, Taniguchi S, Mataga N. Time-resolved
Stokes shift in proteins with
continuum model: Slow dynamics
in proteins.
Journal of Photochemistry and
Photobiology A: Chemistry 2010;215(1):38-45. |
| |
|
| 29. |
Tan TC, Pitsawong
W, WongnateT, Spadiut O, Haltrich
D, Chaiyen P,
Divne C. H-bonding and Positive
Charge at the N(5)/O(4) Locus
are Critical for Covalent Flavin
Attachment in Trametes Pyranose
2-Oxidase.
Journal of Molecular Biology 2010;402(3):578-94
|
| |
|
| 30. |
Nijvipakul S, Ballou
SP, Chaiyen P.
Reduction Kinetics of a Flavin
Oxidoreductase LuxG from Photobacterium
leiognathi (TH1):Half Sites Reactivity.
Biochemistry 2010;49 (43):9241-8
|
| |
|
| 2011 |
| 31. |
Ruangchan N, Tongsook
C, Sucharitakul J, Chaiyen
P. pH-dependent studies
reveal an efficient hydroxylation
mechanism of the oxygenase component
of p-hydroxyphenylacetate 3-hydroxylase.
Journal
of Biological Chemistry 2011;286(1):223-33. |
| |
|
| 32. |
Sucharitakul J,
Wongnate T, Chaiyen P. Hydrogen peroxide
elimination
from C4A-hydroperoxy-flavin in
a flavoprotein oxidase occurs
through a single proton transfer
from flavin N5 to a peroxide leaving
group. Journal
of Biological Chemistry 2011;286(19):16900-9. |
| |
|
| 33. |
Thotsaporn K, Chenprakhon P, Sucharitakul J,
Mattevi A, Chaiyen P. Stabilization of
C4A-hydroperoxy-flavin in a two-component flavin-dependent
monooxygenase is achieved through interactions at flavin N5 and
C4a atoms. Journal
of Biological Chemistry 2011;286(32):28170-80. |
| |
|
| 34. |
Penpassakarn P, Chaiyen P, Palittapongarnpim
P. Efficient heterologous expression and one-step purification
of fully active c-terminal histidine-tagged uridine
monophosphate kinase from Mycobacterium tuberculosis.
The
Southeast Asian Journal of Tropical Medicine and Public
Health 2011;42(6):1452-1459.
|
| |
|
| 35. |
Wongnate T, Sucharitakul J , and Chaiyen P.
Identification of a catalytic base for sugar oxidation in
pyranose 2-oxidase reaction. Chembiochem
2011;12(17):2577-86.
|
| |
|
| 36. |
Tongsook C, Sucharitakul J, Thotsaporn K, Chaiyen
P. Interactions with the substrate phenolic group
are essential for hydroxylation by the oxygenase component of
p-hydroxyphenylacetate 3-hydroxylase. Journal
of Biological Chemistry 2011;286(52):44491–502.
|
| |
|
| 2012 |
| |
|
| 37. |
Chenprakhon P, Panijpan B, Chaiyen P. An
Experiment Illustrating the Change in Ligand pKa upon Protein
Binding. Journal
of Chemical Education 2012; 89:791−795.
|
| |
|
| 38. |
Chosrowjan H, Taniguchi S, Wongnate T, Sucharitakul J,
Chaiyen P, Tanaka F. Conformational
heterogeneity in pyranose 2-oxidase from Trametes
multicolor revealed by ultrafast fluorescence dynamics.
Journal
of Photochemistry and Photobiology A: Chemistry
2012;234(April 15):44-48.
|
| |
|
| 39. |
Oonnanat W, Sucharitakul J, Chaiyen P,
Yuaniyama J. Crystallization and preliminary X-ray analysis of
the reductase component of p-hydroxyphenylacetate
3-hydroxylase from Acinetobacter baumannii. Acinetobacter
baumannii. Acta Crystallographica Section;
F68:720–723.
|
| |
|
| 40. |
Sucharitakul J, Wongnate T, Montersino S, van Berkel, WJH,
Chaiyen P. Reduction Kinetics of 3-Hydroxybenzoate
6-Hydroxylase from Rhodococcus jostii RHA1. Biochemistry
2012; 51(21):4309-21.
|
| |
|
| 41. |
Somprasong N, Jittawuttipoka T, Duang-nkern J, Romsang, A,
Chaiyen P, Schweizer H, Vattanaviboon P, Mongkolsuk S.
Pseudomonas aeruginosa thiol peroxidase protects against
hydrogen peroxide toxicity and displays atypical patterns of
gene regulation. Journal
of Bacteriology 2012; 194(15):3904-12.
|
| |
|
| 42. |
Sopitthummakhun K, Thongpanchang C, Vilaivan T,
Yuthavong Y, Chaiyen P, Leartsakulpanich U. Plasmodium serine
hydroxymethyltransferase as a potential anti-malarial target:
inhibition studies using improved methods for enzyme production
and assay, Malaria
Journal 2012;11(1):194. |
| |
|
| 43. |
Phongsak T, Sucharitakul J, Thotsaporn K,
Oonanant W, Yuvaniyama J, Svasti J, Ballou DP, Chaiyen P.The
C-terminal domain of 4-hydroxyphenylacetate 3-hydroxylase from
Acinetobacter baumannii is an auto-inhibitory domain. J.
Biol.Chem. 2012 ; 287(31):26213-26222. |
| |
|
| 44. |
Taniguchi S, Chosrowjan H, Wongnate T,
Sucharitakul J, Chaiyen P, Tanaka F.
Ultrafast Fluorescence Dynamics of Flavin Adenine Dinucleotide
in Pyranose 2-Oxidases Variants and Their Complexes with
Acetate: Conformational Heterogeneity with Different Dielectric
Constants.
Journal
of Photochemistry and Photobiology A: Chemistry 2012;
245:33-42. |
| |
|
| 45. |
Chaiyen P, Fraaije M, Mattevi
A. The Enigmatic Reaction of Flavins with Oxygen. Trends in
Biochemical Sciences 2012;37(9):373-80. |
| |
|
| 46. |
Tinikul R, Thotsaporn K, Taveekarn V,
Jitrapakdee S, Chaiyen P. The fusion
Vibrio campbellii luciferase as a eukaryotic gene reporter. Journal
of Biotechnology 162 (2012):pp 346–353. |
| |
|
| 2013 |
| |
|
| 47. |
Tan TC, Spadiut O, Wongnate T, Sucharitakul J,
Krondorfer I, Sygmund C, Haltrich D, Chaiyen P,
Peterbauer CK, Divne C*. The 1.6 Å crystal structure of
pyranose dehydrogenase from Agaricus
meleagris rationalizes substrate Specificity and
Reveals a Flavin Intermediate. PLoS
One 2013;8(1):e53567. |
| |
|
| 48. |
Prongjit M, Sucharitakul J, Palfey BA,
Chaiyen P*. Oxidation mode of pyranose
2-oxidase is controlled by pH. Biochemistry 2013
Feb 26;52(8):1437-45. |
| |
|
| 49. |
Wongnate T, Chaiyen P*. The
substrate oxidation mechanism of pyranose 2-oxidase and other
related enzymes in the glucose-methanol-choline
superfamily. FEBS J 2013
Jul;280(13):3009-27. (Review) |
| |
|
| 50. |
Tinikul R, Pitsawong W, Sucharitakul J,
Nijvipakul S, Ballou DP, Chaiyen P*. The
transfer of reduced FMN from LuxG oxidoreductase to luciferase
occurs via free diffusion. Biochemistry 2013
Oct 1;52(39):6834-6843. |
| |
|
| 51. |
Sucharitakul J*, Tongsook C, Pakotiprapha D, van
Berkel WJ, Chaiyen P. The Reaction Kinetics of
3-Hydroxybenzoate 6-Hydroxylase from Rhodoccocus jostii RHA1
Provide an Understanding of the para-Hydroxylation Enzyme
Catalytic Cycle. J Biol Chem.
2013 Dec 6;288:35210-35221. |
| |
|
| 2014 |
| |
|
| 52. |
Wongnate T, Surawatanawong P, Visitsatthawong S,
Sucharitakul J, Scrutton NS, Chaiyen P.
Proton-coupled Electron Transfer and Adduct Configuration are
Important for C4a-Hydroperoxyflavin Formation and Stabilization
in a Flavoenzyme. J. Am.
Chem. Soc. 2014; 136 (1): pp 241–253. |
| |
|
| 53. |
Chitnumsub P, Ittarat P, Jaruwat A,
Noytanom K, Amornwatcharapong W, Pornthanakasem W,
Chaiyen P, Yuthavong Y, Leartsakulpanich
U. Structure of Plasmodium falciparum serine
hydroxymethyltransferase reveals a novel redox switch that
regulates its activities. Acta
Crystallogr D Biol Crystallogr. 2014 Jun;70(Pt
6):1517-27. |
| |
|
| 54. |
Pinthong C, Maenpuen S, Amornwatcharapong W,
Yuthavong Y, Leartsakulpanich U, Chaiyen
P.Distinct Biochemical Properties of Human Serine
Hydroxymethyltransferase Compared to the Plasmodium Enzyme:
Implications for Selective Inhibition. FEBS J. 2014
Jun;281(11):2570-83. |
|
| 55. |
Sucharitakul J, Tinikul R, Chaiyen
P. Mechanisms of Reduced Flavin Transfer in the
Two-component flavin-dependent Monooxygenases. Arch
Biochem Biophys. 2014 Aug;555-556:33-46 |
| |
|
| 56. |
Srisook T, Vongsetskula T*, Sucharitakul J,
Chaiyen P, Tangboriboonrat P. Immobilization of
3-hydroxybenzoate 6-hydroxylase onto functionalized electrospun
polycaprolactone ultrafine fibers: a novel heterogeneous
catalyst. Reactive
and Functional Polymers, 82, 41-46. |
| |
|
| 57. |
Chenprakhon P , Trisrivirat D, Thotsaporn K ,
Sucharitakul J, and Chaiyen P* Control of C4a-hydroperoxyflavin
protonation in the oxygenase component of p-hydroxyphenyl
acetate-3-hydroxylase. Biochemistry 2014
Jul 1;53(25):4084-6. (Rapid Report) |
| |
|
| 58. |
Chitnumsub, P., Jaruwat, A., Riangrungroj, P.,
Ittarat, W., Noytanom, K., Oonanant, W., Vanichthanankul, J.,
Chuankhayan, P., Maenpuen, S., Chen, C.-J., Chaiyen, P.,
Yuthavong, Y., Leartsakulpanich, U.
Structures of Plasmodium vivax serine hydroxymethyltransferase:
Implications for ligand-binding specificity and functional
control
(2014) Acta Crystallographica Section D: Biological
Crystallography, 70 (12), pp. 3177-3186. |
| |
|
| 59. |
Sahin, S., Wongnate, T., Chaiyen, P., Yu, E.H.
Glucose oxidation using oxygen resistant pyranose-2-oxidase for
biofuel cell applications
(2014) Chemical
Engineering Transactions, 41 (Special Issue), pp. 367-372.
|
| 2015 |
| |
|
| 60. |
Messiha HL, Wongnate T, Chaiyen
P, Jones AR, Scrutton NS. Magnetic field effects as a
result of the radical pair mechanism are unlikely in redox
enzymes. J R
Soc Interface. 2015;12(103). |
| |
|
| 61. |
Panatdasirisuk, W., Vongsetskul, T.,
Sucharitakul, J., Chaiyen, P., Tangboriboonrat,
P. Functionalized electrospun regenerated cellulose fibers for
immobilizing pyranose 2-oxidase. Reactive and Functional Polymers
2015;86:47-51. |
| |
|
| 62. |
Luanloet T, Sucharitakul J and Chaiyen
P*. Selectivity of Substrate Binding and Ionization
of 2-Methyl-3-Hydroxypyridine-5-Carboxylic Acid Oxygenase.
FEBS J. 2015;
282(16):3107-25. |
| |
|
| 63. |
Elkhal C.K., Kean K, Parsonage D, Maenpuen S,
Chaiyen P, Claiborne A and Karplus P.A.
Structure and proposed mechanism of alpha-glycerophosphate
oxidase from Mycoplasma pneumonia. FEBS J. 2015;
282(16):3030-42. |
| |
|
| 64. |
Maenpuen S, Amornwatcharapong W, Krasatong P,
Sucharitakul J, Palfey BA, Yuthavong Y, Chitnumsub P,
Leartsakulpanich U, Chaiyen P. Kinetic
mechanism and the rate-limiting step of Plasmodium vivax serine
hydroxymethyltransferase. Journal of
Biological Chemistry 2015;290(13):8656-65. |
| |
|
| 65. |
Maenpuen S, Watthaisong P, Supon P, Sucharitakul
J, Parsonage D, Karplus PA, Claiborne A, Chaiyen
P. Kinetic Mechanism of L-alpha-Glycerophosphate
Oxidase from Mycoplasma pneumoniae. FEBS J. 2015;
282(16):3043-59. |
| |
|
| 66. |
Witschel MC, Rottmann M, Schwab
A, Leartsakulpanich U, Chitnumsub P, Seet M, Tonazzi S,
Schwertz G, Stelzer F, Mietzner T, McNamara C, Thater F,
Freymond C, Jaruwat A, Pinthong C, Riangrungroj P, Oufir M,
Hamburger M, Mäser P, Sanz-Alonso LM, Charman S, Wittlin
S, Yuthavong Y, Chaiyen P, Diederich F.
Inhibitors of plasmodial serine hydroxymethyltransferase
(SHMT): Cocrystal structures of pyrazolopyrans with potent
blood- and liver-stage activities. Journal of
Medicinal Chemistry 2015;58(7):3117-30. |
| |
|
| 67. |
Dhammaraj T, Phintha A, Pinthong C, Medhanavyn D,
Tinikul R, Chenprakhon P, Sucharitakul J, Vardhanabhuti N,
Jiarpinitnun C, and Chaiyen P.
p-Hydroxyphenylacetate 3-hydroxylase as a biocatalyst for the
synthesis of trihydroxyphenolic acids. ACS
Catalysis 2015; 5:4492�4502. |
| |
|
| 68. |
Visitsatthawong S, Chenprakhon P, Chaiyen
P, and Surawatanawong P. Mechanism of oxygen
activation in a flavin-dependent monooxygenase: A nearly
barrierless C4a-hydroperoxyflavin formation via Proton-coupled
electron transfer. Journal of the American Chemical Society 2015;
137(29):9363-74. |
| |
|
| 69. |
Chaiyen P, Scrutton NS. Special Issue:
Flavins and Flavoproteins: Introduction.
FEBS J. 2015
Aug;282(16):3001-2.
|
| |
|
| 70. |
Graf M, Sucharitakul J, Bren U, Chu B, Koellensberger G; Hann
S, Furtmüller P, Obinger C, Peterbauer C, Oostenbrink C.
Chaiyen P, Haltrich, D. Reaction of pyranose
dehydrogenase from Agaricus meleagris with its carbohydrate
substrates. FEBS J. 2015
Nov;282(21):4218-41.
|
| |
|
| 2016 |
| |
|
| 71. |
Sucharitakul J, Medhanavyn D, Pakotiprapha D, van Berkel W.
Chaiyen P. Tyr217 and His213 are Important
for Substrate Binding and Hydroxylation of 3-Hydroxybenzoate
6-Hydroxylase from Rhodococcus jostii. FEBS J 2016
Mar;283(5):860-81.
|
| |
|
| 72. |
Tinikul R and Chaiyen P. Structure,
Mechanism, and Mutation of Bacterial Luciferase In: Advances
in Biochemical Engineering/Technology (Springer-Verlag
Berlin Heidelberg) 2016; 154:47-74.
|
| |
|
| 73. |
Dhammaraj T, Pinthong C, Visitsatthawong S, Tongsook C,
Surawatanawong P, Chaiyen P. A Single-site
Mutation at Ser146 Expands the Reactivity of the Oxygenase
Component of p-Hydroxyphenylacetate 3-Hydroxylase. ACS
Chem Biol. 2016;11(10):2889-2896.
|
| |
|
| 74. |
Thotsaporn K, Tinikul R, Maenpuen S, Phonbuppha J,
Watthaisong P, Chenprakhon P, and Chaiyen P.
Enzymes in the p-hydroxyphenylacetate degradation pathway of
Acinetobacter baumannii. Journal
of Molecular Catalysis B: Enzymatic 134 (2016)
353–366.
|
| |
|
| 2017 |
| |
|
| 75. |
Pruksatrakul T, PPhoopraintra P, Wilairat P, Chaiyen
P, Chantiwas R. Development of a sequential
injection-liquid microextraction procedure with GC-FID for
analysis of short-chain fatty acids in palm oil mill effluent.
Talanta
2017; 165 (1): 612-618.
|
| |
|
| 76. |
Pornsuwan S, Maenpuen S, Kamutira P, Watthaisong P,
Thotsaporn K, Tongsook C, Juttulapa M, Nijvipakul S,
Chaiyen P. 3,4-Dihydroxyphenylacetate
2,3-dioxygenase from Pseudomonas aeruginosa: An
Fe(II)-containing enzyme with fast turnover. PLOS
ONE 2017; February (3): 1-25.
|
| |
|
| 77. |
Pimviriyakul P, Thotsaporn K, Sucharitakul J and
Chaiyen P. Kinetic Mechanism of the
Dechlorinating Flavin-Dependent Monooxygenase HadA. J Biol
Chem. 2017 Mar 24;292(12):4818-4832
|
| |
|
| 78. |
Chenprakhon P, Dhammaraj T, Chantiwas R, Chaiyen
P. Hydroxylation of 4-hydroxyphenylethylamine
derivatives by R263 variants of the oxygenase component of
p-hydroxyphenylacetate-3-hydroxylase. Arch
Biochem Biophys. 2017 Apr 15;620:1-11.
|
| |
|
| 79. |
Schwertz G, Witschel MC, Rottmann M, Bonnert R,
Leartsakulpanich U, Chitnumsub P, Jaruwat A, Ittarat W,
Schäfer A, Aponte RA, Charman SA, White KL, Kundu A,
Sadhukhan S, Lloyd M, Freiberg GM, Srikumaran M, Siggel M,
Zwyssig A, Chaiyen P, Diederich F.
Antimalarial Inhibitors Targeting Serine
Hydroxymethyltransferase (SHMT) with in Vivo Efficacy and
Analysis of their Binding Mode Based on X-ray Cocrystal
Structures.
J Med
Chem. 2017 Jun 22;60(12):4840-4860.
|
| |
|
| 80. |
Amornwatcharapong W, Maenpuen S, Chitnumsub P,
Leartsakulpanich P, and Chaiyen P. Human and
Plasmodium Serine Hydroxymethyl Transferases Differ in
Rate-limiting Steps and pH-Dependent Substrate Inhibition
Behavior. Archives of
Biochemistry and Biophysics 630 (2017), 91-100.
|
| |
|
| 81. |
Pinthong C , Phoopraintra P, Chantiwas R, Pongtharangkul T,
Chenprakhon P, and Chaiyen P. Green and
Sustainable Biocatalytic Production of
3,4,5-Trihydroxycinnamic Acid from Palm Oil Mill Effluent. Process
Biochemistry. 2017; 63: 122-129.
|
| |
|
| 82. |
Schwertz G, Frei MS, Witschel MC, Rottmann M,
Leartsakulpanich U, Chitnumsub P, Jaruwat A, Ittarat A,
Schäfer A, Aponte RA, Trapp N, Mark K, Chaiyen
P, Diederich F. Conformational Aspects in the
Design of Inhibitors for Serine Hydroxymethyltransferase
(SHMT): Biphenyl, Aryl Sulfonamide, and Aryl Sulfone Motifs,
Chemistry - A
European Journal. 2017; 23(57): 14345-14357.
|
| |
|
| 2018 |
| 83. |
Sawasdee K, Sucharitakul J, Dhammaraj T, Niamsiri N,
Chaiyen P, Prapainop K
Encapsulation of the reductase component of
p-Hydroxyphenylacetate Hydroxylase in poly
(lactide-co-glycolide) nanoparticles by three different
emulsification techniques. IET
Nanobiotechnology. 2018 Jun;12(4):423-428. .
|
| |
|
| 84. |
Tinikul R, Chenprakhon P, Maenpuen S, Chaiyen
P. Biotransformation of Plant-Derived Phenolic
Acids. Phenolic
Acids. Biotechnol J. 2018 Jun;13(6):e1700632.
|
| |
|
| 85. |
şahin S, Wongnate T, Chuaboon L, Chaiyen
P, Yu EH. Enzymatic fuel cells with an oxygen
resistant variant of pyranose-2-oxidase as anode biocatalyst.
Biosens
Bioelectron. 2018. pp 17-25.
|
| |
|
| 86. |
Teanphonkrang S, Janke S, Chaiyen P,
Sucharitakul J, Suginta W, Khunkaewla P, Schuhmann W, Ruff A,
Schulte A. Tuned Amperometric Detection of Reduced
β-Nicotinamide Adenine Dinucleotide by Allosteric
Modulation of the Reductase Component of the
p-Hydroxyphenylacetate Hydroxylase Immobilized within a Redox
Polymer.Analytical
Chemistry, Anal. Chem., 2018, 90 (9), 5703-5711. .
|
| |
|
| 87. |
Schwertz G, Witschel MC, Rottmann M,
Leartsakulpanich U, Chitnumsub P, Jaruwat A, Amornwatcharapong
W, Ittarat W, Schäfer A, Aponte RA, Chaiyen
P, and Diederich F. Potent Inhibitors for Plasmodial
Serine Hydroxymethyltransferase (SHMT) Featuring a Spirocyclic
Scaffold, ChemMedChem.
2018 May 8;13(9):931-943. |
| |
|
| 88. |
Phonbuppha J, Maenpuen S, Munkajohnpong P,
Chaiyen P, Tinikul R. A selective determination
of the catalytic cysteine pKa of 2-cysteine succinic
semialdehyde dehydrogenase from Acinetobacter baumannii using
burst kinetics and enzyme adduct formation, FEBS J. 2018
Jul;285(13):2504-2519. |
| |
|
| 89. |
Yuenyaw A, Petchyam N, Kamonsutthipaijit N, Chaiyen
P, Pakotiprapha D. Crystal structure of the flavin
reductase of Acinetobacter baumannii p-hydroxyphenylacetate
3-hydroxylase (HPAH) and identification of amino acid residues
underlying its regulation by aromatic ligands. Archives of
Biochemistry and Biophysics. 2018 Sep 1; 653:24-38.
|
| |
|
| 90. |
Pimviriyakul P, Surawatanawong P, Chaiyen P.
Oxidative Dehalogenation and Denitration by a Flavin-dependent
Monooxygenase is Controlled by Substrate Deprotonation. Chemical
Science. 2018 (9); 7468 – 7482.
|
| |
|
| 91. |
Pimviriyakul P, Chaiyen P. A complete
bioconversion cascade for dehalogenation and denitration by
bacterial flavin-dependent enzymes. J Biol
Chem. 2018 Nov 30;293(48):18525-18539.
|
| |
|
| 2019 |
| 92. |
Chenprakhon P, Wongnate T, Chaiyen P.
Monooxygenation of aromatic compounds by flavin-dependent
monooxygenases. Protein Sci. 2019
Jan;28(1):8-29.
|
| |
|
| 93. |
Chuaboon L, Wongnate T, Punthong P, Kiattisewee C, Lawan N,
Hsu CY, Lin CH, Bornscheuer U, Chaiyen P.
One-Pot Bioconversion of L-Arabinose to L-Ribulose in an
Enzymatic Cascade. Angew Chem Int
Ed Engl. 2019 Feb 18;58(8):2428-2432.
|
| |
|
| 94. |
Wongnate T, Surawatanawong P, Chuaboon L, Lawan N,
Chaiyen P. The mechanism of sugar C-H bond
oxidation by a flavoprotein oxidase occurs by a hydride
transfer before proton abstraction. Chemistry.
2019 Mar 21;25(17):4460-4471.
|
| |
|
| 95. |
Teanphonkrang S, Ernst A, Janke S, Chaiyen
P, Sucharitakul J, Suginta W, Khunkaewla P,
Schuhmann W, Schulte A, Ruff A. Amperometric detection of the
urinary disease biomarker p-HPA by allosteric modulation of a
redox polymer-embedded bacterial reductase. ACS Sens.
2019 May 24;4(5):1270-1278.
|
| |
|
| 96. |
Duangpummet P, Chaiyen P, Chenprakhon P.
Lipase-Catalyzed Esterification: An Inquiry-Based Laboratory
Activity to Promote High School Students' Understanding and
Positive Perceptions of Green Chemistry. J Chem
Educ. 2019 May;96(6):1205-1211.
|
| |
|
| 97. |
Ubonprasert S, Jaroensuk J, Pornthanakasem W,
Kamonsutthipaijit N, Wongpituk P, Mee-Udorn P, Rungrotmongkol
T, Ketchart O, Chitnumsub P, Leartsakulpanich U,
Chaiyen P, Maenpuen S. A flap motif in human
serine hydroxymethyl transferase is important for structural
stabilization, ligand binding, and control of product release.
J Biol
Chem. 2019 Jul 5;294(27):10490-10502.
|
| |
|
| 98. |
Jaroensuk J, Intasian P, Kiattisewee C, Munkajohnpon P,
Chunthaboon P, Buttranon S, Trisrivirat D, Wongnate T,
Maenpuen S, Tinikul R, Chaiyen P. Addition of
formate dehydrogenase increases the production of renewable
alkane from an engineered metabolic pathway. J Biol Chem. 2019 Jul 26;294(30):11536-11548.
|
| |
|
| 99. |
Pongpamorn P, Watthaisong P, Pimviriyakul P, Jaruwat A, Lawan N, Chitnumsub P, Chaiyen P. Identification of a Hotspot Residue for Improving the Thermostability of a Flavin-Dependent Monooxygenase. Chembiochem. 2019 Dec 13;20(24):3020-3031.
|
| |
|
| 100. |
Watthaisong P, Pongpamorn P, Pimviriyakul P, Maenpuen S,
Ohmiya Y, Chaiyen P. A Chemo-Enzymatic
Cascade for the Smart Detection of Nitro- and Halogenated
Phenols. Angew Chem Int Ed Engl. 2019 Sep;58(38):13254-13258
- Highlighted as "Hot
Paper:Biocatalysis" and "Frontispiece Article"
- Highlighted
in more than 8 news outlets
|
| 101. |
Jaroensuk J, Wong YH, Zhong W, Liew CW, Maenpuen S, Sahili AE, Atichartpongkul S, Chionh YH, Nah Q, Thongdee N, McBee ME, Prestwich EG, DeMott MS, Chaiyen P, Mongkolsuk S, Dedon P, Lescar J, Fuangthong M. Crystal structure and catalytic mechanism of the essential m1G37 tRNA methyltransferase TrmD from Pseudomonas aeruginosa. RNA. 2019 Nov;25(11):1481-1496.
|
| |
|
| 102. |
Thongdee N, Jaroensuk J, Atichartpongkul S, Chittrakanwong J, Chooyoung K, Srimahaeak T, Chaiyen P, Vattanaviboon P, Mongkolsuk S, Fuangthong M. TrmB, a tRNA m7G46 methyltransferase, plays a role in hydrogen peroxide resistance and positively modulates the translation of katA and katB mRNAs in Pseudomonas aeruginosa. Nucleic Acids Res. 2019 Sep;47(17):9271-9281.
|
| |
|
| 103. |
Songsungthong W, Yongkiettrakul S, Bohan LE, Nicholson ES, Prasopporn S, Chaiyen P, Leartsakulpanich U. Diaminoquinazoline MMV675968 from Pathogen Box inhibits Acinetobacter baumannii growth through targeting of dihydrofolate reductase. Sci Rep. 2019 Oct;9(1):15625.
|
| |
|
| 2020 |
| 104. |
Pimviriyakul P, Wongnate T, Tinikul R, Chaiyen P. Microbial degradation of halogenated aromatics: molecular mechanisms and enzymatic reactions. Microb Biotechnol. 2020;13(1):67–86.
|
| |
|
| 105. |
Jaroensuk J, Intasian P, Wattanasuepsin W, Akeratchatapan N, Kesornpun C, Kittipanukul N, Chaiyen P. Enzymatic reactions and pathway engineering for the production of renewable hydrocarbons. J Biotechnol. 2020 Feb 10;309:1-19.
|
| |
|
| 106. |
Phonbuppha J, Tinikul R, Wongnate T, Intasian P, Hollmann F, Paul CE, Chaiyen P. A Minimized Chemoenzymatic Cascade for Bacterial Luciferase in Bioreporter Applications. Chembiochem 2020 Jul;21(14):2073-79.
|
| |
|
| 107. |
Pitsawong W, Chenprakhon P, Dhammaraj T, Medhanavyn D, Sucharitakul J, Tongsook C, van Berkel WJH, Chaiyen P, Miller AF. Tuning of pKa values activates substrates in flavin-dependent aromatic hydroxylases. J Biol Chem. 2020 Mar 20;295(12):3965-3981.
|
| |
|
| 108. |
Maenpuen S, Pongsupasa V, Pensook W, Anuwan P, Kraivisitkul N, Pinthong C, Phonbuppha J, Luanloet T, Wijma HJ, Fraaije MW, Lawan N, Chaiyen P, Wongnate T. Rational-design engineering to create the flavin reductase variants with thermostable and solvent-tolerant properties. ChemBioChem. 2020;21(10):1481-1491.
|
| |
|
| 109. |
Munkajohnpong P, Kesornpun C, Buttranon S, Jaroensuk J, Weeranoppanant N, Chaiyen P. Fatty Alcohol Production: An Opportunity of Bioprocess. Biofuel Bioprod Bioref 2020;14:986-1009.
|
| |
|
| 110. |
Woraruthai T, Kunno J, Pongsopon M, Yansakon K, Phoopraintra P, Chantiwas R, Leartsakulpanich U, Chaiyen P, Wongnate T. Chaiyen P. Identification and Cultivation of Hydrogenotrophic Methanogens from Palm Oil Mill Effluent for High Methane Production. Int J Energy Res 2020;44:10058-70.
|
| |
|
| 111. |
Trisrivirat D, Lawan N, Chenprakhon P, Matsui D, Asano Y, Chaiyen P. Mechanistic insights into the dual activities of the single active site of L-lysine oxidase/monooxygenase from Pseudomonas sp. AIU 813. J Biol Chem. 2020 Aug 7;295(32):11246-11261.
|
| |
|
| 112. |
Pimviriyakul P, Chaiyen P. Overview of flavin-dependent enzymes. Enzymes. 2020;47:1-36.
|
| |
|
| 113. |
Chenprakhon P, Pimviriyakul P, Tongsook C, Chaiyen P. Phenolic hydroxylases. Enzymes. 2020; 47:283-326.
|
| |
|
| 114. |
Phintha A, Prakinee K, Chaiyen P. Structures, mechanisms and applications of flavin-dependent halogenases. Enzymes. 2020;47:327-364.
|
| |
|
| 115. |
Pimviriyakul P, Chaiyen P. Flavin-dependent dehalogenases. Enzymes. 2020;47:365-397.
|
| |
|
| 116. |
Chaiyen P, Tamanoi F. Preface: Flavin-Dependent Enzymes: Mechanisms, Structures and Applications. Enzymes. 2020;47:xv-xvi.
|
| |
|
| 117. |
Patchsung M, Jantarug K, Pattama A, Aphicho K, Suraritdechachai S, Meesawat P, Sappakhaw K, Leelahakorn N, Ruenkam T, Wongsatit T, Athipanyasilp N, Eiamthong B, Lakkanasirorat B, Phoodokmai T, Niljianskul N, Pakotiprapha D, Chanarat S, Homchan A, Tinikul R, Kamutira P, Phiwkaow K, Soithongcharoen S, Kantiwiriyawanitch C, Pongsupasa V, Trisrivirat D, Jaroensuk J, Wongnate T, Maenpuen S, Chaiyen P, Kamnerdnakta S, Swangsri J, Chuthapisith S, Sirivatanauksorn Y, Chaimayo C, Sutthent R, Kantakamalakul W, Joung J, Ladha A, Jin X, Gootenberg JS, Abudayyeh OO, Zhang F, Horthongkham N, Uttamapinant C. Clinical validation of a Cas13-based assay for the detection of SARS-CoV-2 RNA. Nat Biomed Eng. 2020;4(12):1140-9.
|
| |
|
| 118. |
Trisrivirat D, Hughes JMX, Hoeven R, Faulkner M, Toogood H, Chaiyen P, Scrutton NS. Promoter engineering for microbial bio-alkane gas production. Synth Biol (Oxf). 2020 Oct 27;5(1):ysaa022.
|
| |
|
| 2021 |
| 119. |
Sucharitakul J, Buttranon S, Wongnate T, Chowdhury NP, Prongjit M, Buckel W, Chaiyen P. Modulations of the reduction potentials of flavin-based electron bifurcation complexes and semiquinone stabilities are key to control directional electron flow. FEBS J 2021 Feb;288(3):1008-26.
|
| |
|
| 120. |
Phintha A, Prakinee K, Jaruwat A, Lawan N, Visitsatthawong S, Kantiwiriyawanitch C, Songsungthong W, Trisrivirat D, Chenprakhon P, Mulholland AJ, van Pee KH, Chitnumsub P, Chaiyen P. Dissecting the low catalytic capability of flavin-dependent halogenases. J Biol Chem. 2021 Jan;296:100068.
|
| |
|
| 121. |
Sucharitakul J, Buckel W, Chaiyen P. Rapid kinetics reveal surprising flavin chemistry in the bifurcating electron transfer flavoprotein from Acidaminococcus fermentans. J Biol Chem. 2021;296:100124.
|
| |
|
| 122. |
Kraithong T, Sucharitakul J, Buranachai C, Jeruzalmi D, Chaiyen P, Pakotiprapha D. Real-time investigation of the roles of ATP hydrolysis by UvrA and UvrB during DNA damage recognition in nucleotide excision repair. DNA Repair (Amst). 2021 Jan;97:103024.
|
| |
|
| 123. |
Tinikul R, Lawan N, Akeratchatapan N, Pimviriyakul P, Chinantuya W, Suadee C, Sucharitakul J, Chenprakhon P, Ballou DP, Entsch B, Chaiyen P. Protonation status and control mechanism of flavin–oxygen intermediates in the reaction of bacterial luciferase. FEBS J, in press.
|
| |
|
| 124. |
Pongpamorn P, Kiattisewee C, Kittipanukul N, Jaroensuk J, Trisrivirat D, Maenpuen S, Chaiyen P. Carboxylic Acid Reductase Can Catalyze Ester Synthesis in Aqueous Environments. Angew Chem Int Ed Engl 2021 Mar;60(11):5749-53.
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Chapter in Book
|
|
1.
|
Chaiyen P,
Suadee C, Meevoothisom V. Degradation
of phenylacetic acid by bacteria
from Thai soil. In: Annual Report
of ICBiotech. Vol. 21. Osaka:
International Center for Biotechnology;
1998. p. 862-7.
|
| |
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2.
|
Wongnate T and Chaiyen, P. Pyranose
oxidases. In: Hille R., Miller, S.M., Palfey, B. (Eds.).
Handbook of Flavoproteins. Oxidases, dehydrogenases and
related systems. Volume 1. 2013. p. 177-193.
|
| |
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3.
|
Tinikul R and Chaiyen P. Structure, Mechanism, and Mutation
of Bacterial Luciferase In: Advances in Biochemical
Engineering/Technology (Springer-Verlag Berlin Heidelberg)
2016; 154:47-74.
|
| |
|
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4.
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Maenpuen S, Tinikul S, Chenprakhon P and Chaiyen P.
Production of Valuable Phenolic Compounds from Lignin by
Biocatalysis: State-of-the-Art Perspective. In Emerging Areas
in Bioengineering (2018), Wiley-VCH
Verlag GmbH & Co. pp 102-124.
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8 chapters in books published by Walter de Gruyter GmbH, Springer-Verlag Berlin-Heidelberg, Wiley-VCH Verlag GmbH & Co, and Elsevier.
Serial Volume Editor of the Book: The Enzymes, Volume 47.
Flavin-Dependent Enzymes to be published on 1st October 2020
|
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Patent :
|
| 2555 |
ยีนสำหรับฟิวชั่นลูซิเฟอเรสเอนไซม์จากเชื้อแบคทีเรีย Vibrio
campbellii
ที่เสถียรต่อภาวะอุณหภูมิสูงและองค์ประกอบของน้ำยาสำหรับวัดแอคติวิตี้เอนไซม์ลูซิเฟอเรส
|
สิทธิบัตร
(1201000720) |
| 2559 |
Fusion bacterial luciferase gene, reporter vector, and assay kit |
Patent Cooperation Treaty, PCT (PCT/TH/2016000051) |
| 2561 |
องค์ประกอบเครื่องหมายโปรตีน (Protein Marker) |
อนุสิทธิบัตร (เลขที่คำขอ 1503001543 เลขที่อนุสิทธิบัตร 14519) |
| |
องค์ประกอบเครื่องหมายโปรตีนที่ติดสี (Pre-stained Protein
Marker) |
อนุสิทธิบัตร (เลขที่คำขอ 1503001542 เลขที่อนุสิทธิบัตร 13888) |
|
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Papers
in Proceedings
|
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1.
|
Chaiyen P,
Suadee C, Thotsaporn K, Svasti
J. Studies
of the two-component p-hydroxyphenylacetate
hydroxylase from Acinetobacter
baumannii. In: Chapman
S, Perham R, Scrutton N, editors.
Flavins and Flavoproteins 2002:
Proceedings of the Fourteenth
International Symposium; 2002
July 14-18; St. John's College,
University of Cambridge, Uk.
Berlin: Agency for Scientific
Publications; 2002. p. 975-80.
|
|
2.
|
Sucharitakul J, Chaiyen
P, Ballou DP, Massey
V. Probing
the mechanism of 2-methyl-3-hydroxypyridine-5-carboxylic
acid oxygenase by using 8-substituted-FAD
analogs. In: Chapman S,
Perham R, Scrutton N, editors.
Flavins and Flavoproteins 2002:
Proceedings of the Fourteenth
International Symposium; 2002
July 14-18; St. John's College,
University of Cambridge, Uk.
Berlin: Agency for Scientific
Publications; 2002. p. 381-6.
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|
3.
|
Sucharitakul J, Chaiyen
P, Entsch B, Ballou
DP. Mechanistic
studies on the reductase component
of p-hydroxyphenylacetate
3-hydroxylase from Acinetobacter
baumannii.. In: Nishino
T, Miura R, Tanokura M, Fukui
K, editors. Flavins and Flavoproteins
2005: Proceedings of the Fifteenth
International Symposium; 2005
April 17-22; Shonan Village
Center, Hayama, Japan: ARchiTect
Inc.; 2005. p. 275-80.
|
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4.
|
Chaiyen P,
Phongsak T, Sucharitakul J,
Ballou DP. Review
and perspectives of how reduced
flavin is transferred between
the reductase and oxygenase
p-hydroxyphenylacetate 3-hydroxylase.
In: Frago S, Gomez-moreno C,
Medina M, editors. Flavins and
Flavoproteins 2008: Proceedings
of the Sixteenth International
Symposium; 2008 June 8-13; Jaca,
Spain: Prensas Universitarias
de Zaragoza; 2008. p. 27-44.
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|
|
Invited Lectures (Overseas)
|
|
1.
|
Oral Presentation
"p-Hydroxyphenylacetic
acid hydroxylase from Acinetobacter
baumannii" 15th the Federation
of Asian and Oceanian Biochemists
and Molecular Biologists (FAOBMB)
Symposium, Beijing, China, October
21-24, 2001. |
| |
|
|
2.
|
Invited Seminar Lecture
"Reduced Flavin Transfer
between Components of p-Hydroxyphenyl-
acetate 3-Hydroxylase". A
seminar at University of Pavia,
Italy, June 12, 2006. |
| |
|
|
3.
|
Invited Symposium Lecture
"How the reductase and oxygenase
work together in a two-component
flavoenzyme, p-hydroxyphenylacetate".
16th
International Symposium on Flavins
and Flavoproteins, Jaca, Spain
, Jun 10, 2008. |
| |
|
| 4. |
Invited
Speaker
Gordon Research Conference 2010:
Enzymes, Co-enzymes & Metabolic
Pathways "What is necessary
for C4a-hydroperoxy-FAD formation
in pyranose 2-oxidase reacion?"
July 19-23, 2010 at Waterville
Valley Resort, New Hampshire,
USA. |
| |
|
| 5. |
Invited seminar lecture
" Exploring the Reaction
Mechanism of Pyranose 2-Oxidase
Through Transient Kinetics and
Site-Directed Mutagenesis"
at Wake Forest University, July
27, 2010, Winston-Salem, North
Carolina, USA. |
| |
|
| 6. |
Invited Symposium lecture
“Oxygenation mechanism of p-hydroxyphenylacetate
hydroxylase, a two-component flavin-dependent
monooxygenase”. The 3rd Asia Pacific Protein Association
(APPA) Conference, May 6-9, Shanghai, China. |
| |
|
| 7. |
Invited Symposium lecture
“Use of Kinetic Isotope Effects and Transient Kinetics to
Unravel the Mechanism of H2O2 Elimination from
C4a-Hydroperoxy-flavin in Pyranose2-Oxidase”. The IXth
European Symposium of The Protein Society. May 22-26, 2011,
Stockholm, Sweden. |
| |
|
| 8. |
Invited Symposium lecture
“Understanding reaction mechanisms of two-component
flavin-dependent monooxygenases through a model of
p-hydroxyphenylacetate hydroxylase”. 17th International
Conference on Cytochrome P450, at The University of Manchester,
UK 26th to 30th of June, 2011. |
| |
|
| 9. |
Oral Presentation
Mechanistic Studies of Flavin- and PLP-Dependent Enzymes.
Presentation by TWAS young affiliate at 22nd General Meeting at
International centre in Theroretical Physics, Trieste, Italy, on
21-23 November 2011. |
| |
|
| 10. |
Invited Speaker
Gordon Research Conference 2012: Biocatalysis "
Biocatalysis by Flavoenzymes:From Enzyme Mechanisms to
Rational Engineering”
July 8-13, 2012, Bryant University, Smithfield, RI, USA.
|
| |
|
| 11. |
Invited Symposium Lecture
"Control and Versatility in Catalysis by Flavin-dependent
Enzymes"
Enzyme Engineering XXII: Emerging Topics in Enzyme
Engineering
September 22-26, 2013, Toyama International Conference Center,
Toyama, Japan
|
| |
|
| 12. |
Invited Lecture
"Bacterial Luciferase from Thailand as a Gene
Reporter"
at BioMedical Research Institute, AIST, Tsukuba, Ibaraki,
Japan
|
| |
|
| 13. |
Invited Lecture
"Aromatic flavin-dependent monooxygenase as a
regio-specific biocatalyst"
May 12, 2014 at Institute of Biochemistry, University of
Greifswald, Greifswald, Mecklenburg-Vorpommern,
Germany.
|
| |
|
| 14. |
Invited Lecture
“Oxygen Activation by Flavin-Dependent Enzymes”
May 14, 2014 at The Max Planck Institute (MPI) for Terrestrial
Microbiology, Marburg, Germany.
|
| |
|
| 15. |
Invited Lecture
Invited Lecture “Mechanistic Studies of Flavin- and
PLP-dependent Enzymes”
at BASF-The Chemical Company, Ludwigshafen,
Germany.
|
| |
|
| 16. |
Invited Lecture
"Mechanisms of Oxygen Activation by Flavin-Dependent Enzymes"
October 22, 2014 at 15th IUBMB - 24th
FAOBMB-TSBMB International Conference, Academia Sinica,
Taipei, Taiwan
|
| |
|
| 17. |
Invited Lecture
"Mechanism and Biocatalysis of Flavin-Dependent
Oxygenases"
13 November, 2014 at 9th Joint Conference on
Chemistry Program
Semarang, Indonesia.
|
| |
|
| 18. |
Invited Lecture
Gordon Research Conference 2015: Enzymes, Co-enzymes &
Metabolic Pathways "Oxygenation and Beyond by
Two-component Flavin-dependent monooxygenases" July
12-17, 2015 at Waterville Valley Resort, New Hampshire, USA.
|
| |
|
| 19. |
Invited Lecture
“Biotransformation of Aromatic Compounds by
Flavin-Dependent Monooxygenases” July 26 – 30,
2015 at the 12th Biotrans, Vienna, Austria
|
| |
|
| 20. |
Invited Lecture
"Two-component Flavin-Dependent Monooxygenases:
From Aromatic Hydroxylation to Light-Emitting Reaction"
September 16, 2015
at BioMedical Research Institute, AIST, Tsukuba, Ibaraki,
Japan
|
| |
|
| 21. |
Invited Lecture
“From Mechanisms to Application of
Two-component Flavin-Dependent Monooxygenases” Sep
19-20, 2015 at a regional meeting of Japan Society for
Bioscience, Biotechnology and Agrochemistry, Toyama
Prefectural University, Toyama, Japan
|
| |
|
| 22. |
Invited Lecture
"Two-component
Flavin-Dependent Monooxygenases: Challenge and Opportunity"
Oct 9, 2015 at BallouFest Symposium 2015 in honor of Professor
Dr. David P. Ballou, Department of Biological Chemistry,
University of Michigan, Ann Arbor, MI, USA.
|
| |
|
| 23. |
Invited Lecture
"Bacterial Luciferase as a Eukaryotic Reporter
System" at 19th International Symposium on Bioluminescence and
Chemiluminescence, May 29 - June 2, Tsukuba, Japan.
|
| |
|
| 24. |
Invited Lecture
(Plenary Lecture) "Oxygenation and Beyond by
Two-Component Flavin-Dependent Monooxygenases"
21th BEST Conference June 24-25, 2016, National Central
University, Taoyuan, Taiwan.
|
| |
|
| 25. |
Invited Lecture
"From Mechanistic Understanding to Applications of
Two-Component
Flavin-Dependent Monooxygenases" at 8th European Meeting on
OxiZymes, OxiZymes 2016, 3-6 July, 2016, The Netherlands.
|
| |
|
| 26. |
Invited Lecture
(Plenary Lecture) " Flavin-Dependent
Monooxygenases:One Intermediate for Many Reactions " The Fifth
International Conference on Cofactors (ICC-05) and Active
Enzyme Molecule 2016 (AEM 2016)” September 4 to 8, 2016,
Unazuki, Toyama, Japan.
|
| |
|
| 27. |
Invited Lecture
"Dehalogenase and Pyranose 2-oxidases and their
applications" September 9, 2016 at R&D Headquarter, Amano
Enzyme, Gifu, Japan.
|
| |
|
| 28. |
Invited Lecture
“Flavin-Dependent Monooxygenases for
Sustainable Technology” University of
Bristol, Department of Chemistry, UK, March 2017
|
| |
|
| 29. |
Invited Lecture
“Flavin-Dependent Monooxygenases for
Sustainable Technology” University of Manchester,
Manchester Institute of Biotechnology, UK, March 2017
|
| |
|
| 30. |
Invited Lecture
“Flavin-Dependent Monooxygenases for
Sustainable Technology” Laboratory of Molecular Biology
(LMB), Cambridge, UK, March 2017
|
| |
|
| 31. |
Invited Lecture
(Plenary Lecture) "Insights into Mechanisms of
Flavin-Dependent Monooxygenases
and Beyond " 19th International Symposium on Flavins and
Flavoproteins,
Groningen, The Netherlands, July 2-6, 2017
|
| |
|
| 32. |
Invited Lecture
"Beyond Monooxygenation by Flavin-Dependent
Enzymes " 20th International Conference on Cytochrome
P450: Biochemistry, Biophysics and Biotechnology
Düsseldorf, 27-31 August 2017
|
| |
|
| 33. |
Invited Symposium Lecture
" From Fundamental to Novel Detection Technology of
Flavin-Dependent Monooxygenases" 2018 Protein Science Society
of Japan Annual Meeting, 26-28 June 2018, Niigata, Japan.
|
| |
|
| 34. |
Invited Lecture
"From Fundamental Enzyme Catalysis To Applications in
Detection and Green Chemistry" at Institute for Integrated
Cell-Material Sciences (iCeMS), 29 June 2018, Kyoto, Japan
|
| |
|
| 35. |
Invited Symposium Lecture
"Novel Enzymatic and Cascade Reactions for
Biocatalysis and Biodetection"at EMBO workshop: Enzymes,
biocatalysis and chemical biology: The new frontiers, 09 - 12
September 2018 Pavia, Italy, University of Pavia, Italy 9-12
September 2018
|
| |
|
| 36. |
Invited Symposium Lecture
"Flavin-dependent dehalogease and halogenase"at 25
Year Biochemistry Workshopt at Technical University Dresden,
Dresden, Germany, 28 September 2018
|
| |
|
| 37. |
Invited Lecture
"Versatile Reactions of Two-Component Flavin-Dependent
Monooxygenases" at Department of Experimental Biology &
RECETOX, Faculty of Science, Masaryk University, Brno, Czech
Republic, 1 November 2018
|
| |
|
| 38. |
Invited Lecture
"Versatile Reactions of Two-Component Flavin-Dependent
Monooxygenases" at Department of Biochemistry, Wageningen
University, Wageningen, The Netherlands, 2 November 2018
|
| |
|
| 39. |
Invited Lecture
"Flavin-Dependent Enzymes: Mechanisms and Innovations" at
Department of Chemistry, University of California, Berkeley,
CA, USA 23 April 2019.
|
| |
|
| 40. |
Distinguished Alumni Lecture
"Flavin-Dependent Enzymes: Mechanisms and Innovations" at
Department of Biological Chemistry, University of Michigan,
Ann Arbor, USA 25 April 2019.
|
| |
|
| 41. |
Keynote Lecture
"Novel Enzymatic Cascades for Biodetection, Biocatalysis and
Biofuel" at Southeast Asia Catalysis Conference (SACC),
National University of Singapore, Singapore, 23-24 May, 2019
|
| |
|
| 42. |
Invited Lecture
"Enzymatic Cascades for Biocatalysis, Biodetection and
Biofuel" at Biotrans 2019, Groningen, The Netherlands. July
7-11, 2019.
|
| |
|
| 43. |
Invited Lecture
27th FAOBMB & 44th MSBMB Conference and IUBMB Special
Symposia, Berjaya Times Square Hotel, Kuala Lumpur, Malaysia.
19-22 August 2019.
|
| |
|
| 44. |
Invited Lecture
"Microbial enzymatic systems for converting agro-industrial
waste to useful compounds" at International Symposium on the
Genetics of Industrial Microorganisms (GIM 2019), Pisa, Italy,
8-11 September 2019.
|
| |
|
| 45. |
Invited Lecture
"Biodetoxification, Biosynthesis and Bioluminescence from
Phenolic Toxicants: Three applications in a One-Pot Reaction"
at An Annual Symposium of Japan Association for
Bioluminescence and Chemiluminescence (JABC). Tsukuba, Japan.
5 October 2019.
|
| |
|
| 46. |
Invited Lecture
at Multi-step Enzyme Catalyzed Processes Conference
(MECP2020), Aachen, Germany. 30 March – 2 April, 2020.
|
| |
|
| 47. |
Invited Lecture
at the World Conference on Protein Science, Sapporo, Japan.
7-10 July, 2020.
|
| |
|
|
|
|
Invited Symposium Lectures in Thailand
|
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> 80 times |
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