{"id":5778,"date":"2023-06-19T11:17:53","date_gmt":"2023-06-19T04:17:53","guid":{"rendered":"https:\/\/science.mahidol.ac.th\/muscsynergy\/?p=5778"},"modified":"2025-07-14T15:58:48","modified_gmt":"2025-07-14T08:58:48","slug":"imb-lab-3","status":"publish","type":"post","link":"https:\/\/science.mahidol.ac.th\/muscsynergy\/imb-lab-3\/","title":{"rendered":"IMB LAB"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"5778\" class=\"elementor elementor-5778\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-6b4ea787 elementor-section-full_width elementor-section-stretched elementor-section-height-default elementor-section-height-default\" data-id=\"6b4ea787\" data-element_type=\"section\" data-e-type=\"section\" data-settings=\"{&quot;stretch_section&quot;:&quot;section-stretched&quot;,&quot;background_background&quot;:&quot;classic&quot;,&quot;shape_divider_bottom&quot;:&quot;mountains&quot;}\">\n\t\t\t\t\t\t\t<div class=\"elementor-background-overlay\"><\/div>\n\t\t\t\t\t\t<div class=\"elementor-shape elementor-shape-bottom\" aria-hidden=\"true\" data-negative=\"false\">\n\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 1000 100\" preserveAspectRatio=\"none\">\n\t<path class=\"elementor-shape-fill\" opacity=\"0.33\" d=\"M473,67.3c-203.9,88.3-263.1-34-320.3,0C66,119.1,0,59.7,0,59.7V0h1000v59.7 c0,0-62.1,26.1-94.9,29.3c-32.8,3.3-62.8-12.3-75.8-22.1C806,49.6,745.3,8.7,694.9,4.7S492.4,59,473,67.3z\"\/>\n\t<path class=\"elementor-shape-fill\" opacity=\"0.66\" d=\"M734,67.3c-45.5,0-77.2-23.2-129.1-39.1c-28.6-8.7-150.3-10.1-254,39.1 s-91.7-34.4-149.2,0C115.7,118.3,0,39.8,0,39.8V0h1000v36.5c0,0-28.2-18.5-92.1-18.5C810.2,18.1,775.7,67.3,734,67.3z\"\/>\n\t<path class=\"elementor-shape-fill\" d=\"M766.1,28.9c-200-57.5-266,65.5-395.1,19.5C242,1.8,242,5.4,184.8,20.6C128,35.8,132.3,44.9,89.9,52.5C28.6,63.7,0,0,0,0 h1000c0,0-9.9,40.9-83.6,48.1S829.6,47,766.1,28.9z\"\/>\n<\/svg>\t\t<\/div>\n\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-1336bd88\" data-id=\"1336bd88\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-68abc4bc elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"68abc4bc\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-5759f731\" data-id=\"5759f731\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-6f6c19e5 elementor-widget elementor-widget-heading\" data-id=\"6f6c19e5\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">IMB Lab<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-7c7af144 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"7c7af144\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-aa0332b\" data-id=\"aa0332b\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-149c12bf elementor-widget elementor-widget-heading\" data-id=\"149c12bf\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Industrial Microbiology &amp; Biotechnology (IMB) Laboratory.<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-23a43c93 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"23a43c93\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-42ceb25d\" data-id=\"42ceb25d\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-6dcd0aaf elementor-widget-divider--view-line elementor-widget elementor-widget-divider\" data-id=\"6dcd0aaf\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"divider.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-divider\">\n\t\t\t<span class=\"elementor-divider-separator\">\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-32f213f7 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"32f213f7\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-103e3b23\" data-id=\"103e3b23\" data-element_type=\"column\" data-e-type=\"column\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-41ae6c0 elementor-widget elementor-widget-image\" data-id=\"41ae6c0\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"300\" height=\"450\" src=\"https:\/\/science.mahidol.ac.th\/muscsynergy\/wp-content\/uploads\/2023\/06\/messageImage_1752479739212.jpg\" class=\"attachment-large size-large wp-image-6817\" alt=\"\" srcset=\"https:\/\/science.mahidol.ac.th\/muscsynergy\/wp-content\/uploads\/2023\/06\/messageImage_1752479739212.jpg 300w, https:\/\/science.mahidol.ac.th\/muscsynergy\/wp-content\/uploads\/2023\/06\/messageImage_1752479739212-200x300.jpg 200w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-714b17f elementor-widget elementor-widget-icon-box\" data-id=\"714b17f\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"icon-box.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-icon-box-wrapper\">\n\n\t\t\t\n\t\t\t\t\t\t<div class=\"elementor-icon-box-content\">\n\n\t\t\t\t\t\t\t\t\t<h3 class=\"elementor-icon-box-title\">\n\t\t\t\t\t\t<span  >\n\t\t\t\t\t\t\tAssoc.Prof. Thunyarat Pongtharangkul\t\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/h3>\n\t\t\t\t\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-2fdd9a3\" data-id=\"2fdd9a3\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-40fdd249 elementor-icon-list--layout-traditional elementor-list-item-link-full_width elementor-widget elementor-widget-icon-list\" data-id=\"40fdd249\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"icon-list.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<ul class=\"elementor-icon-list-items\">\n\t\t\t\t\t\t\t<li class=\"elementor-icon-list-item\">\n\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-icon\">\n\t\t\t\t\t\t\t<svg aria-hidden=\"true\" class=\"e-font-icon-svg e-fas-phone-alt\" viewBox=\"0 0 512 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M497.39 361.8l-112-48a24 24 0 0 0-28 6.9l-49.6 60.6A370.66 370.66 0 0 1 130.6 204.11l60.6-49.6a23.94 23.94 0 0 0 6.9-28l-48-112A24.16 24.16 0 0 0 122.6.61l-104 24A24 24 0 0 0 0 48c0 256.5 207.9 464 464 464a24 24 0 0 0 23.4-18.6l24-104a24.29 24.29 0 0 0-14.01-27.6z\"><\/path><\/svg>\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-text\">Tel. (66+)086-379-7037<\/span>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t<li class=\"elementor-icon-list-item\">\n\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-icon\">\n\t\t\t\t\t\t\t<svg aria-hidden=\"true\" class=\"e-font-icon-svg e-fas-mail-bulk\" viewBox=\"0 0 576 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M160 448c-25.6 0-51.2-22.4-64-32-64-44.8-83.2-60.8-96-70.4V480c0 17.67 14.33 32 32 32h256c17.67 0 32-14.33 32-32V345.6c-12.8 9.6-32 25.6-96 70.4-12.8 9.6-38.4 32-64 32zm128-192H32c-17.67 0-32 14.33-32 32v16c25.6 19.2 22.4 19.2 115.2 86.4 9.6 6.4 28.8 25.6 44.8 25.6s35.2-19.2 44.8-22.4c92.8-67.2 89.6-67.2 115.2-86.4V288c0-17.67-14.33-32-32-32zm256-96H224c-17.67 0-32 14.33-32 32v32h96c33.21 0 60.59 25.42 63.71 57.82l.29-.22V416h192c17.67 0 32-14.33 32-32V192c0-17.67-14.33-32-32-32zm-32 128h-64v-64h64v64zm-352-96c0-35.29 28.71-64 64-64h224V32c0-17.67-14.33-32-32-32H96C78.33 0 64 14.33 64 32v192h96v-32z\"><\/path><\/svg>\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-text\">e-mail : thunyarat.pon@mahidol.ac.th<\/span>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t<li class=\"elementor-icon-list-item\">\n\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-icon\">\n\t\t\t\t\t\t\t<svg aria-hidden=\"true\" class=\"e-font-icon-svg e-fas-map-marker-alt\" viewBox=\"0 0 384 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M172.268 501.67C26.97 291.031 0 269.413 0 192 0 85.961 85.961 0 192 0s192 85.961 192 192c0 77.413-26.97 99.031-172.268 309.67-9.535 13.774-29.93 13.773-39.464 0zM192 272c44.183 0 80-35.817 80-80s-35.817-80-80-80-80 35.817-80 80 35.817 80 80 80z\"><\/path><\/svg>\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-text\">Location: R3\/6 SC3 Bldg., Salaya Campus, Faculty of Science, Mahidol University<\/span>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t<\/ul>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-1d3af4b elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"1d3af4b\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-7807a43\" data-id=\"7807a43\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-ab94a5c elementor-section-full_width elementor-section-stretched elementor-section-height-default elementor-section-height-default\" data-id=\"ab94a5c\" data-element_type=\"section\" data-e-type=\"section\" data-settings=\"{&quot;stretch_section&quot;:&quot;section-stretched&quot;,&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-5e862162\" data-id=\"5e862162\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-2bbf3d72 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"2bbf3d72\" data-element_type=\"section\" data-e-type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-64d32ee4\" data-id=\"64d32ee4\" data-element_type=\"column\" data-e-type=\"column\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-fdf3e14 elementor-tabs-view-horizontal elementor-widget elementor-widget-tabs\" data-id=\"fdf3e14\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"tabs.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-tabs\">\n\t\t\t<div class=\"elementor-tabs-wrapper\" role=\"tablist\" >\n\t\t\t\t\t\t\t\t\t<div id=\"elementor-tab-title-2661\" class=\"elementor-tab-title elementor-tab-desktop-title\" aria-selected=\"true\" data-tab=\"1\" role=\"tab\" tabindex=\"0\" aria-controls=\"elementor-tab-content-2661\" aria-expanded=\"false\">Isolation & characterization of bacteria in biological sample<\/div>\n\t\t\t\t\t\t\t\t\t<div id=\"elementor-tab-title-2662\" class=\"elementor-tab-title elementor-tab-desktop-title\" aria-selected=\"false\" data-tab=\"2\" role=\"tab\" tabindex=\"-1\" aria-controls=\"elementor-tab-content-2662\" aria-expanded=\"false\">Biodegradation & waste treatment<\/div>\n\t\t\t\t\t\t\t\t\t<div id=\"elementor-tab-title-2663\" class=\"elementor-tab-title elementor-tab-desktop-title\" aria-selected=\"false\" data-tab=\"3\" role=\"tab\" tabindex=\"-1\" aria-controls=\"elementor-tab-content-2663\" aria-expanded=\"false\">Bioconversion & bio-based chemicals<\/div>\n\t\t\t\t\t\t\t\t\t<div id=\"elementor-tab-title-2664\" class=\"elementor-tab-title elementor-tab-desktop-title\" aria-selected=\"false\" data-tab=\"4\" role=\"tab\" tabindex=\"-1\" aria-controls=\"elementor-tab-content-2664\" aria-expanded=\"false\">Bench-scale bioreactor cultivation (Bacteria & Yeast)<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t\t<div class=\"elementor-tabs-content-wrapper\" role=\"tablist\" aria-orientation=\"vertical\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-tab-title elementor-tab-mobile-title\" aria-selected=\"true\" data-tab=\"1\" role=\"tab\" tabindex=\"0\" aria-controls=\"elementor-tab-content-2661\" aria-expanded=\"false\">Isolation & characterization of bacteria in biological sample<\/div>\n\t\t\t\t\t<div id=\"elementor-tab-content-2661\" class=\"elementor-tab-content elementor-clearfix\" data-tab=\"1\" role=\"tabpanel\" aria-labelledby=\"elementor-tab-title-2661\" tabindex=\"0\" hidden=\"false\"><p><img decoding=\"async\" class=\"alignnone size-full wp-image-5200\" src=\"https:\/\/science.mahidol.ac.th\/muscsynergy\/wp-content\/uploads\/2022\/06\/isolation.jpg\" alt=\"\" width=\"800\" height=\"290\" srcset=\"https:\/\/science.mahidol.ac.th\/muscsynergy\/wp-content\/uploads\/2022\/06\/isolation.jpg 800w, https:\/\/science.mahidol.ac.th\/muscsynergy\/wp-content\/uploads\/2022\/06\/isolation-300x109.jpg 300w, https:\/\/science.mahidol.ac.th\/muscsynergy\/wp-content\/uploads\/2022\/06\/isolation-768x278.jpg 768w\" sizes=\"(max-width: 800px) 100vw, 800px\" \/><\/p><p>Our expertise includes isolation of bacteria from biological samples (e.g. food, soil, wastewater, biofilm). Pure culture of each isolate can be identified to species level using 16s rDNA sequencing with necessary biochemical tests (depending on the species of interest). Number of viable cells and spores in the sample can be quantified using a spread plate technique. In case that the microbial populations (microbiome) is of interest, a culture-independent analysis using Next-generation sequencing (NGS) can be employed. This technique allows for discovering of microbes that are \u2018non-culturable\u2019 or may be in relatively low abundance and therefore \u2018not detectable\u2019 using traditional methods.<\/p><ul><li>Isolation of bacteria from biological sample (12,000 THB\/sample)<\/li><li>Identification and characterization of bacterial isolate using 16s rDNA sequencing and biochemical tests (2,100 THB\/isolate)<\/li><li>Quantification of bacterial viable cell and\/or endospore in biological sample (600 THB\/sample)<\/li><li>Evaluation of bacterial community (microbiome) in biological sample using Next Generation Sequencing (NGS) (18,000 THB\/sample<\/li><\/ul><p>Our publications:<\/p><ul><li>Wongbunmak A, Khiawjan S, Suphantharika M, Pongtharangkul T. BTEX- and naphthalene-degrading bacterium Microbacterium esteraromaticum strain SBS1-7 isolated from estuarine sediment. Journal of Hazardous Materials 2017;339:82-90.<\/li><li>Khiawjan S, Suphantharika M, Pongtharangkul T. 2014. Isolation of BTEX degrading bacteria from petroleum contaminated seabed sludges. Proceedings of the 2nd ASEAN Plus Three Graduate Research Congress (2nd AGRC), February 5-7. Bangkok, Thailand.<\/li><li>Leiim J, Suphantharika M, Pongtharangkul T. 2015. Isolation and characterization of xylenes degrading-bacteria, Pseudomonas citronellolis PC1. Proceedings of The 27th Annual Meeting of the Thai Society for Biotechnology and International Conference, November 18-19. Bangkok, Thailand.<\/li><li>Prasitwuttisak, W, Sapsanyakorn, K, Ruanglek, V and Pongtharangkul, T. (2018) A study on effects of 1,3-butadiene exposure on bacterial community in activated sludge using next generation sequencing. The 186th The IIER International Conference, 26-27 August 2018, Taipei, Taiwan.<\/li><\/ul><\/div>\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-tab-title elementor-tab-mobile-title\" aria-selected=\"false\" data-tab=\"2\" role=\"tab\" tabindex=\"-1\" aria-controls=\"elementor-tab-content-2662\" aria-expanded=\"false\">Biodegradation & waste treatment<\/div>\n\t\t\t\t\t<div id=\"elementor-tab-content-2662\" class=\"elementor-tab-content elementor-clearfix\" data-tab=\"2\" role=\"tabpanel\" aria-labelledby=\"elementor-tab-title-2662\" tabindex=\"0\" hidden=\"hidden\"><p><img decoding=\"async\" class=\"size-full wp-image-5204 aligncenter\" src=\"http:\/\/localhost\/2022\/\/wp-content\/uploads\/2022\/06\/Biodegradation_new.jpg\" alt=\"\" width=\"800\" height=\"290\" srcset=\"https:\/\/science.mahidol.ac.th\/muscsynergy\/wp-content\/uploads\/2022\/06\/Biodegradation_new.jpg 800w, https:\/\/science.mahidol.ac.th\/muscsynergy\/wp-content\/uploads\/2022\/06\/Biodegradation_new-300x109.jpg 300w, https:\/\/science.mahidol.ac.th\/muscsynergy\/wp-content\/uploads\/2022\/06\/Biodegradation_new-768x278.jpg 768w\" sizes=\"(max-width: 800px) 100vw, 800px\" \/><\/p><p>We have experiences on extraction and quantification of hydrocarbons (in liquid and gas phase) using chromatography techniques. Biodegradation test using either a pure bacterial culture or natural consortium can be performed in parallel with an appropriate control experiment in order to evaluate the potential or efficiency of the culture\/consortium of interest. An ability of the culture\/consortium to form biofilm on various solid supports can be evaluated in order to support the decision making on the supporting material. The structure of the biofilm of interest can be visualized using a scanning electron microscope (SEM).<\/p><ul><li>Biodegradation test for hydrocarbons (12,000 THB\/sample)<\/li><li>Biofilm formation test (6,000 THB\/sample)<\/li><li>Determination of biofilm structure using SEM (18,000 THB\/sample)<\/li><\/ul><p>Our publications:<\/p><ul><li>Wongbunmak A, Khiawjan S, Suphantharika M, Pongtharangkul T. BTEX- and naphthalene-degrading bacterium Microbacterium esteraromaticum strain SBS1-7 isolated from estuarine sediment. Journal of Hazardous Materials 2017;339:82-90.<\/li><li>Khiawjan S, Suphantharika M, Pongtharangkul T. 2014. Isolation of BTEX degrading bacteria from petroleum contaminated seabed sludges. Proceedings of the 2nd ASEAN Plus Three Graduate Research Congress (2nd AGRC), February 5-7. Bangkok, Thailand.<\/li><li>Leiim J, Suphantharika M, Pongtharangkul T. 2015. Isolation and characterization of xylenes degrading-bacteria, Pseudomonas citronellolis PC1. Proceedings of The 27th Annual Meeting of the Thai Society for Biotechnology and International Conference, November 18-19. Bangkok, Thailand.<\/li><\/ul><\/div>\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-tab-title elementor-tab-mobile-title\" aria-selected=\"false\" data-tab=\"3\" role=\"tab\" tabindex=\"-1\" aria-controls=\"elementor-tab-content-2663\" aria-expanded=\"false\">Bioconversion & bio-based chemicals<\/div>\n\t\t\t\t\t<div id=\"elementor-tab-content-2663\" class=\"elementor-tab-content elementor-clearfix\" data-tab=\"3\" role=\"tabpanel\" aria-labelledby=\"elementor-tab-title-2663\" tabindex=\"0\" hidden=\"hidden\"><p><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-5208 aligncenter\" src=\"http:\/\/localhost\/2022\/\/wp-content\/uploads\/2022\/06\/Bioconversion_new.jpg\" alt=\"\" width=\"800\" height=\"290\" srcset=\"https:\/\/science.mahidol.ac.th\/muscsynergy\/wp-content\/uploads\/2022\/06\/Bioconversion_new.jpg 800w, https:\/\/science.mahidol.ac.th\/muscsynergy\/wp-content\/uploads\/2022\/06\/Bioconversion_new-300x109.jpg 300w, https:\/\/science.mahidol.ac.th\/muscsynergy\/wp-content\/uploads\/2022\/06\/Bioconversion_new-768x278.jpg 768w\" sizes=\"(max-width: 800px) 100vw, 800px\" \/><\/p><p>Our lab focuses on the utilization of biocatalyst, including a crude enzyme extract, purified enzyme and whole-cell, for environmental friendly and sustainable production of bio-based chemicals. The target compound-of-interest can be extracted from the reaction mixture or biological sample, and then identified\/quantified using various chromatography techniques.<\/p><ul><li>Extraction of target compound in biological sample (3,600 THB\/sample)<\/li><li>Identification of target compound in biological sample using chromatography techniques (15,000 THB\/sample)<\/li><li>Quantification of target compound in biological sample using chromatography techniques (2,400-5,400 THB\/sample)<\/li><\/ul><p>Our publications:<\/p><ul><li>Wangrangsimagul N, Klinsakul K, Vangnai AS, Wongkongkatep J, Inprakhon P, Honda K, Ohtake H, Kato J, Pongtharangkul T. Bioproduction of vanillin using an organic solvent-tolerant Brevibacillus agri Applied Microbiology and Biotechnology 2012 Jan;93(2):555-63.<\/li><li>Siriphongphaew A, Pisnupong P, Wongkongkatep J, Inprakhon P, Vangnai AS, Honda K, Ohtake H, Kato J, Ogawa J, Shimizu S, Urlacher VB, Schmid RD, Pongtharangkul T. Development of a whole-cell biocatalyst co-expressing P450 monooxygenase and glucose dehydrogenase for synthesis of epoxyhexane. Applied Microbiology and Biotechnology 2012 Jul;95(2):357-67.<\/li><li>Sungwallek S, Payongsri P, Chaiyen P, Pongtharangkul T. 2016. Development of a whole-cell biocatalyst for caffeic acid production. Proceedings of The 28th Annual Meeting of the Thai Society of Biotechnology and International Conference, November 28-30. Chiang Mai, Thailand.<\/li><\/ul><\/div>\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-tab-title elementor-tab-mobile-title\" aria-selected=\"false\" data-tab=\"4\" role=\"tab\" tabindex=\"-1\" aria-controls=\"elementor-tab-content-2664\" aria-expanded=\"false\">Bench-scale bioreactor cultivation (Bacteria & Yeast)<\/div>\n\t\t\t\t\t<div id=\"elementor-tab-content-2664\" class=\"elementor-tab-content elementor-clearfix\" data-tab=\"4\" role=\"tabpanel\" aria-labelledby=\"elementor-tab-title-2664\" tabindex=\"0\" hidden=\"hidden\"><p><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-5215 aligncenter\" src=\"http:\/\/localhost\/2022\/\/wp-content\/uploads\/2022\/06\/Bench-scale.jpg\" alt=\"\" width=\"600\" height=\"280\" srcset=\"https:\/\/science.mahidol.ac.th\/muscsynergy\/wp-content\/uploads\/2022\/06\/Bench-scale.jpg 600w, https:\/\/science.mahidol.ac.th\/muscsynergy\/wp-content\/uploads\/2022\/06\/Bench-scale-300x140.jpg 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p><p>Our expertise includes cultivation of natural and recombinant bacteria (or yeast) for production of cell, endospore as well as target product (e.g. alcohol, organic acid, protein). Fed-batch and repeated-batch cultivation can also be employed for higher productivity.<\/p><ul><li>Bioreactor cultivation conditions for maximum productivity (18,000-30,000 THB\/batch)<\/li><\/ul><p><strong>Our Publications:<\/strong><\/p><ul><li>Pongtharangkul T, Chuekitkumchorn P, Suwanampa N, Payongsri P, Honda K, Panbangred W. Kinetic properties and stability of glucose dehydrogenase from Bacillus amyloliquefaciens SB5 and its potential for cofactor regeneration. AMB Express 2015; 5;68.<\/li><li>Suwanpatcharakul M, Pakdeecharoema C, Visuttitewina S, Pesirikan N, Chauvatcharin S, Pongtharangkul T. Process Optimization for an industrial-scale production of Diphtheria toxin by Corynebacterium diphtheriae PW8. Biologicals 2016; 44(6):534-9.<\/li><\/ul><\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-9cd5103 elementor-section-full_width elementor-section-stretched elementor-section-height-default elementor-section-height-default\" data-id=\"9cd5103\" data-element_type=\"section\" data-e-type=\"section\" data-settings=\"{&quot;stretch_section&quot;:&quot;section-stretched&quot;,&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-6d137d62\" data-id=\"6d137d62\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-64162acb elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"64162acb\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-55ebf302\" data-id=\"55ebf302\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-5eb41e34 elementor-widget elementor-widget-heading\" data-id=\"5eb41e34\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">IMB Lab -- Industrial Microbiology &amp; Biotechnology (IMB) Laboratory<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>IMB Lab Industrial Microbiology &amp; Biotechnology (IMB) Laboratory. Assoc.Prof. Thunyarat Pongtharangkul Tel. (66+)086-379-7037 e-mail : thunyarat.pon@mahidol.ac.th Location: R3\/6 SC3 Bldg., Salaya Campus, Faculty of Science, Mahidol University Isolation &#038; characterization of bacteria in biological sample Biodegradation &#038; waste treatment Bioconversion &#038; bio-based chemicals Bench-scale bioreactor cultivation (Bacteria &#038; Yeast) Isolation &#038; characterization of bacteria in [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":5828,"comment_status":"open","ping_status":"open","sticky":false,"template":"elementor_header_footer","format":"standard","meta":{"footnotes":""},"categories":[7],"tags":[],"class_list":["post-5778","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-services-solutions"],"_links":{"self":[{"href":"https:\/\/science.mahidol.ac.th\/muscsynergy\/wp-json\/wp\/v2\/posts\/5778","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/science.mahidol.ac.th\/muscsynergy\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/science.mahidol.ac.th\/muscsynergy\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/science.mahidol.ac.th\/muscsynergy\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/science.mahidol.ac.th\/muscsynergy\/wp-json\/wp\/v2\/comments?post=5778"}],"version-history":[{"count":0,"href":"https:\/\/science.mahidol.ac.th\/muscsynergy\/wp-json\/wp\/v2\/posts\/5778\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/science.mahidol.ac.th\/muscsynergy\/wp-json\/wp\/v2\/media\/5828"}],"wp:attachment":[{"href":"https:\/\/science.mahidol.ac.th\/muscsynergy\/wp-json\/wp\/v2\/media?parent=5778"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/science.mahidol.ac.th\/muscsynergy\/wp-json\/wp\/v2\/categories?post=5778"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/science.mahidol.ac.th\/muscsynergy\/wp-json\/wp\/v2\/tags?post=5778"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}