|
ประวัติและผลงาน
(Curriculum Vitae) |
|
การศึกษา
: |
พ.ศ. 2528 |
มัธยมศึกษาตอนต้น
โรงเรียนสตรีภูเก็ต |
พ.ศ. 2531 |
มัธยมศึกษาตอนปลาย
โรงเรียนหาดใหญ่วิทยาลัย |
พ.ศ. 2535 |
ปริญญาตรี วิทยาศาสตรบัณฑิต
(เกียรตินิยมอันดับ 1) สาขาเคมี
มหาวิทยาลัยสงขลานครินทร์ |
พ.ศ. 2540 |
ปริญญาเอก สาขาชีวเคมี มหาวิทยาลัยมิชิแกน
แอนน์อาร์เบอร์ ประเทศสหรัฐอเมริกา |
|
การทำงาน
: |
พ.ศ.
2560-ปัจจุบัน |
Professor and Head, School of Biomolecular Science and Engineering, Vidyasirimedhi Institute of Science and Technology (VISTEC) |
พ.ศ.
2552-2560 |
ศาสตราจารย์
ภาควิชาชีวเคมี คณะวิทยาศาสตร์
มหาวิทยาลัยมหิดล |
พ.ศ.
2548-2552 |
รองศาสตราจารย์
ภาควิชาชีวเคมี คณะวิทยาศาสตร์
มหาวิทยาลัยมหิดล |
พ.ศ.
2544 |
ผู้ช่วยศาสตราจารย์
ภาควิชาชีวเคมี คณะวิทยาศาสตร์
มหาวิทยาลัยมหิดล |
พ.ศ.
2540 |
อาจารย์
ภาควิชาชีวเคมี คณะวิทยาศาสตร์
มหาวิทยาลัยมหิดล |
พ.ศ.
2551-2552 |
รองหัวหน้าภาควิชาชีวเคมี
คณะวิทยาศาสตร์ มหาวิทยาลัยมหิดล |
พ.ศ.
2552-2554 |
หัวหน้าภาควิชาชีวเคมี
คณะวิทยาศาสตร์ มหาวิทยาลัยมหิดล |
พ.ศ.
2554-2558 |
รองคณบดีฝ่ายวิจัย คณะวิทยาศาสตร์ มหาวิทยาลัยมหิดล |
พ.ศ.
2536-2539 |
ผู้ช่วยสอน
(Teaching assistant) นักศึกษาระดับปริญญาตรีและบัณฑิตศึกษา
ห้องปฏิบัติการชีวเคมี (BC 416/516)
มหาวิทยาลัยมิชิแกน |
พ.ศ.
2537 |
ผู้ช่วยสอน
(Teaching assistant) นักศึกษาระดับบัณฑิตศึกษา
รายวิชา Enzyme Kinetics (BC 573)
มหาวิทยาลัยมิชิแกน |
พ.ศ.
2559-2560 |
คณะกรรมการบริหาร ศูนย์พันธุวิศวกรรมและเทคโนโลยีชีวภาพแห่งชาติ สำนักงานพัฒนาวิทยาศาสตร์และเทคโนโลยีแห่งชาติ (สวทช.) |
พ.ศ.
2558-ปัจจุบัน |
อนุกรรมาธิการเศรษฐกิจกระแสใหม่ ส่วนคณะกรรมาธิการขับเคลื่อนการปฏิรูปประเทศ ด้านเศรษฐกิจ สภาขับเคลื่อนการปฏิรูปประเทศ |
|
รางวัลเกียรติยศ
: |
พ.ศ.
2528 |
ได้รับพระราชทานรางวัลจากสมเด็จพระเทพรัตน์ราชสุดาฯ
สยามบรมราชกุมารี รางวัลพระราชทานนักเรียนเรียนดี
ระดับมัธยมศึกษาตอนต้น ของกระทรวงศึกษาธิการ |
พ.ศ.
2528-2540 |
ทุนโครงการพัฒนาและส่งเสริมผู้มีความสามารถพิเศษทางวิทยาศาสตร์และเทคโนโลยี
(พสวท.) ตั้งแต่ระดับมัธยมศึกษาตอนปลายจนถึงปริญญาเอก |
พ.ศ.
2532 |
รางวัลผลการศึกษายอดเยี่ยมทางวิทยาศาสตร์
ระดับมหาวิทยาลัย ชั้นปีที่ 1 ของมูลนิธิศาสตราจารย์
ดร. แถบ นีละนิธิ |
พ.ศ.
2536 |
รางวัลผลการศึกษายอดเยี่ยมทางวิทยาศาสตร์
ระดับมหาวิทยาลัย ชั้นปีที่ 4 ของมูลนิธิศาสตราจารย์
ดร. แถบ นีละนิธิ |
พ.ศ.
2538 |
รางวัล
Chrisman Award สำหรับนักศึกษาปริญญาเอกที่มีผลการเรียนผลงานวิจัยดี
จากมหาวิทยาลัยมิชิแกน |
พ.ศ.
2541 |
รางวัล
Murphy Award สำหรับผู้สำเร็จการศึกษาที่มีผลงานตีพิมพ์ดีเยี่ยม
จากมหาวิทยาลัยมิชิแกน |
พ.ศ.
2543 |
Recipient
of Federation of Asian and Oceanic
Biochemistry and Molecular Biology
(FAOBMB) travel fellowship |
พ.ศ.
2546 |
รับพระราชทานทุนวิจัยลอรีอัล
ประเทศไทย เพื่อสตรีในงานวิทยาศาสตร์
(L'OREAL-UNESCO Fellowship for
Woman in Science) ประจำปี 2546
จากทูลกระหม่อมหญิงอุบลรัตนราชกัญญาสิริวัฒนาพรรณวดี |
พ.ศ.
2548 |
รางวัลนักวิทยาศาสตร์รุ่นใหม่
ประจำปี พ.ศ. 2548 (สาขาชีวเคมี)
ของมูลนิธิส่งเสริมวิทยาศาสตร์และเทคโนโลยี
ในพระบรมราชูปถัมภ์ |
พ.ศ.
2551 |
Affiliate
Fellow of TWAS (The Academy of
Sciences for the Developing World) |
พ.ศ.
2552 |
รางวัล
นักชีวเคมีและชีววิทยาโมเลกุลรุ่นใหม่
ประจำปี พ.ศ. 2552 จากสาขาชีวเคมีและชีววิทยาโมเลกุล
สมาคมวิทยาศาสตร์แห่งประเทศไทยในพระราชูปถัมภ์ |
พ.ศ.
2552 |
รางวัลอาจารย์ตัวอย่าง
(ระดับรองศาสตราจารย์) จากสภาอาจารย์คณะวิทยาศาสตร์
ประจำปี พ.ศ. 2552 |
พ.ศ.
2553 |
รางวัลอาจารย์ตัวอย่าง
จากสภาคณาจารย์มหาวิทยาลัยมหิดล
ประจำปี พ.ศ. 2552 |
พ.ศ.
2553 |
รางวัลนักวิจัยดีเด่นสาขาเทคโนโลยีชีวภาพ
กองทุนทะกูชิ ประจำปี 2553 จากมูลนิธิส่งเสริมเทคโนโลยีชีวภาพแห่งประเทศไทย |
พ.ศ.
2553 |
รางวัลTRF-CHE-Scopus
Researcher Award 2010 |
พ.ศ.
2554 |
รางวัลศิษย์เก่าดีเด่นของคณะวิทยาศาสตร์ มหาวิทยาลัยสงขลานครินทร์ และรางวัลศิษย์เก่าดีเด่น (ด้านผลงานเด่น) ของมหาวิทยาลัยสงขลานครินทร์ ประจำปี 2554 |
พ.ศ.
2555 |
รางวัลนักวิจัยดีเด่นแห่งชาติ ประจำปี 2555 สาขาวิทยาศาสตร์เคมีและเภสัช จากสำดิดนักงานคณะกรรมการวิจัยแห่งชาติ (วช.) |
พ.ศ.
2556 |
ได้รับทุนส่งเสริมกลุ่มวิจัย “เมธีวิจัยอาวุโส สกว.” จากสำนักงานกองทุนสนับสนุนการวิจัย |
พ.ศ.
2557 |
Chair of the Organizing Committee, IUBMB 18th International Symposium on Flavins and Flavoproteins |
พ.ศ.
2558 |
ได้รับเชิญเป็นผู้พูดเพื่อสร้างแรงบันดาลใจ ในงาน TEDx Bangkok |
พ.ศ.
2558 |
รางวัลนักวิทยาศาสตร์ดีเด่นประจำปี พ.ศ. 2558 (สาขาชีวเคมี) ของมูลนิธิส่งเสริมวิทยาศาสตร์และเทคโนโลยี ในพระบรมราชูปถัมภ์ |
พ.ศ.
2558 |
ได้รับมอบเข็มทองคำเชิดชูเกียรติฯ นักเรียนทุนรัฐบาลไทยดีเด่น ประจำปี 2558 จาก สมาคมนักเรียนทุนรัฐบาลไทย ร่วมกับ มูลนิธิสมาคมนักเรียนทุนรัฐบาลไทย |
พ.ศ.
2559 |
BioTalk Plenary Awardee from Biotechnology and Biochemical Engineering Society of Taiwan (BEST) |
พ.ศ.
2559 |
โครงการ Bacterial luciferase ได้รับรางวัลที่ 3 โครงการ Tech Planter Thailand ในชื่อทีม Enzmart และรางวัลพิเศษจาก Leave a Nest ให้ไปเข้าร่วมงาน Final Grand Prix ณ ประเทศญี่ปุ่น |
พ.ศ.
2559 |
ได้รับทุนส่งเสริมกลุ่มวิจัย “เมธีวิจัยอาวุโส สกว.” จากสำนักงานกองทุนสนับสนุนการวิจัย (2559-2562) |
พ.ศ.
2560 |
รางวัลผลงานประดิษฐ์คิดค้น ประจำปี ๒๕๖๐ “องค์ประกอบของเครื่องหมายโปรตีน” |
พ.ศ.
2560 |
รางวัลผลงานวิจัยระดับดีมาก ประจำปี ๒๕๖๐ “การศึกษาเอนไซม์เซอรีนไฮดรอกซีเมทิลทรานซเฟอเรสเพื่อเป็นเป้าหมายยาต้านมาลาเรีย” |
พ.ศ.
2560 |
ได้รับอันดับที่ 1 ในการนำเสนอแผนธุรกิจในโปรแกรม “Leaders in Innovation Fellowship” จาก Royal Academy of Engineering and Newton Fund ประเทศสหราชอาณาจักร |
พ.ศ.
2562 |
Distinguished Alumni Lecture, Department of Biological Chemistry,
University of Michigan, Ann Arbor, USA |
2562 |
Outstanding Protein Scientist of Thailand |
พ.ศ.
2563 |
Research Excellence Award for "Pyranose 2-oxidase as an efficient biocatalyst for sugar conversion" from the National Research Council of Thailand (NRCT) |
|
กองบรรณาธิการวารสาร (Editorial Board Members) : |
|
The Journal of Biological Chemistry (JBC) (from July 2012-present) |
|
Archives
of Biochemistry and Biophysics (from November 2012-present) |
|
eLife (from June 2019-present) |
|
|
ผู้ประเมินบทความ
ของวารสารวิชาการระดับนานาชาติ
(Ad Hoc Journal Reviewer) : |
|
ACS Catalysis
|
|
Acta Crystallographica Section F |
|
Applied Environmental Microbiology |
|
Applied Microbiology and Biotechnology |
|
Advanced Synthesis & Catalysis |
|
Archives of Biochemistry and Biophysics |
|
Biochemistry |
|
Biochimie |
|
Biochimica Biophysica Acta |
|
Bioresource Technology |
|
Biotechnology Advances |
|
Biotechnology & Bioengineering |
|
Biotechnology Journal |
|
Chemistry & Biology |
|
ChemCatChem |
|
Chemical Science |
|
FEBS Journal |
|
FEMS Microbiology Letter |
|
Journal of Agricultural and Food Chemistry |
|
The Journal of Biological Chemistry (JBC) |
|
Journal of Chemical Education |
|
Journal of Molecular Catalysis B: Enzymatic |
|
Journal of the American Chemical Society (JACS) |
|
Journal of Photochemistry and Photobiology B: Biology |
|
Microbial Cell Factories |
|
Nature Chemical Biology |
|
PNAS |
|
Trends in Biochemical Science |
|
ผู้ประเมินโครงการ
ของแหล่งเงินทุนวิจัย (Reviewer
of Funding Organizations) : |
|
Austrian
Science Fund |
|
National Science Foundation (NSF, USA.) |
|
สำนักงานกองทุนสนับสนุนการวิจัย
(สกว.) |
|
สำนักงานพัฒนาวิทยาศาสตร์และเทคโนโลยีแห่งชาติ
(สวทช.) |
|
สำนักงานคณะกรรมการการอุดมศึกษา
(สกอ.) |
|
มหาวิทยาลัยมหิดล |
|
สาขางานวิจัยที่สนใจ
: |
|
Enzyme catalysis; Enzyme mechanism; Enzyme engineering
Biocatalysis; Biorefinery; Bioenergy
Biosensor; Bioreporter
Metabolic Engineering; Synthetic Biology
Flavins and Flavoproteins; Aldolase; Redox Enzymes; pyridoxal-5-phosphate enzymes
เอนไซม์
กลไกการทำงานของเอนไซม์
ฟลาวิน; วิตามิน B2
จลหพลศาสตร์ของเอนไซม์
PLP; วิตามิน B6
ชีวเคมีเชิงกายภาพ
ตัวตรวจวัดทางชีวภาพ |
|
หัวข้องานวิจัยที่กำลังดำเนินการ
: |
|
การศึกษากลไกการทำงานของกลุ่มเอนไซม์
ที่ใช้วิตามิน B2 และวิตามิน
B6 ในการทำปฏิกิริยา (Mechanistic
enzymology of flavin-dependent
and pyridoxal 5'-phosphate dependent
enzymes) |
|
ผลงานตีพิมพ์ในระดับนานาชาติ
: |
|
บทความในวารสารวิชาการ |
2540 |
1. |
Chaiyen
P, Brissette P, Ballou
DP, Massey V. Reaction of 2-methyl-3-hydroxypyridine-5-carboxylic
acid (MHPC) oxygenase with N-methyl-5-hydroxynicotinic
acid: Studies on the mode of binding,
and protonation status of the
substrate. Biochemistry
1997;36(45):13856-64. |
|
|
2. |
Chaiyen
P, Ballou DP, Massey
V. Gene cloning, sequence analysis,
and expression of 2-methyl-3-hydroxypyridine-5-carboxylic
acid oxygenase. Proceedings
of the National Academy of Sciences
of the United States of America
1997;94(14):7233-8. |
|
|
3. |
Chaiyen
P, Brissette P, Ballou
DP, Massey V. Unusual mechanism
of oxygen atom transfer and product
rearrangement in the catalytic
reaction of 2-methyl-3-hydroxypyridine-5-carboxylic
acid oxygenase. Biochemistry
1997;36(26):8060-70. |
|
|
4. |
Chaiyen
P, Brissette P, Ballou
DP, Massey V. Thermodynamics and
reduction kinetics properties
of 2-methyl-3- hydroxypyridine-5-carboxylic
acid oxygenase. Biochemistry 1997;36(9):2612-21.
|
|
|
2544 |
5. |
Chaiyen
P, Suadee C, Wilairat
P. A novel two-protein component
flavoprotein hydroxylase p-hydroxyphenylacetate
hydroxylase from Acinetobacter
baumannii. European
Journal of Biochemistry 2001;268(21):5550-61. |
|
|
2547 |
6. |
Chaiyen
P, Sucharitakul J, Svasti
J, Entsch B, Massey V, Ballou
DP. Use of 8-Substituted-FAD Analogues
to Investigate the Hydroxylation
Mechanism of the Flavoprotein
2-Methyl-3-hydroxypyridine-5-carboxylic
Acid Oxygenase. Biochemistry 2004;43(13):3933-43. |
|
|
7. |
Thotsaporn
K, Sucharitakul J, Wongratana
J, Suadee C, Chaiyen P.
Cloning and expression of p-hydroxyphenylacetate
3-hydroxylase from Acinetobacter
baumannii: Evidence of the
divergence of enzymes in the class
of two-protein component aromatic
hydroxylases. Biochimica
et Biophysica Acta - Gene Structure
and Expression 2004;1680(1):60-6. |
|
|
2548 |
8. |
Hommalai
G, Chaiyen P,
Svasti J. Studies on the transglucosylation
reactions of cassava and Thai
rosewood b-glucosidases
using 2-deoxy-2-fluoro-glycosyl-enzyme
intermediates. Archives
of Biochemistry and Biophysics
2005;442(1):11-20. |
|
|
9. |
Oonanant
W, Sucharitakul J, Yuvaniyama
J, Chaiyen P.
Crystallization and preliminary
X-ray crystallographic analysis
of 2-methyl-3-hydroxypyridine-5-carboxylic
acid (MHPC) oxygenase from Pseudomonas
sp. MA-1. Acta
Crystallographica Section F: Structural
Biology and Crystallization Communications 2005;61(3):312-4. |
|
|
10. |
Sucharitakul
J, Chaiyen P,
Entsch B, Ballou DP. The reductase
of p-hydroxyphenylacetate 3-hydroxylase
from Acinetobacter baumannii requires p-hydroxyphenylacetate
for effective catalysis. Biochemistry
2005;44(30):10434-42. |
|
|
2549 |
11. |
Kujawa
M, Ebner H, Leitner C, Hallberg
BM, Prongjit M, Sucharitakul J,
Ludwig R, Rudsander U, Peterbauer
C, Chaiyen P,
Haltrich D, Divne C. Structural
basis for substrate binding and
regioselective oxidation of monosaccharides
at C3 by pyranose 2-oxidase. Journal
of Biological Chemistry 2006;281(46):35104-15. |
|
|
12. |
Sucharitakul
J, Chaiyen P,
Entsch B, Ballou DP. Kinetic mechanisms
of the oxygenase from a two-component
enzyme, p-hydroxyphenylacetate
3-hydroxylase from Acinetobacter
baumannii. Journal
of Biological Chemistry 2006;281(25):17044-53. |
|
|
2550 |
13. |
Alfieri
A, Fersini F, Ruangchan N, Prongjit
M, Chaiyen P,
Mattevi A. Structure of the monooxygenase
component of a two-component flavoprotein
monooxygenase. Proceedings
of the National Academy of Sciences
of the United States of America
2007;104(4):1177-82. |
|
|
14. |
Suadee
C, Nijvipakul S, Svasti J, Entsch
B, Ballou DP, Chaiyen
P. Luciferase from Vibrio
campbellii is more thermostable
and binds reduced FMN better than
its homologues. Journal
of Biochemistry 2007;142(4):539-52. |
|
|
15. |
Sucharitakul
J, Phongsak T, Entsch B, Svasti
J, Chaiyen P,
Ballou DP. Kinetics of a two-component p-hydroxyphenylacetate
hydroxylase explain how reduced
flavin is transferred from the
reductase to the oxygenase. Biochemistry
2007;46(29):8611-23. |
|
|
2551 |
16. |
Nijvipakul
S, Wongratana J, Suadee C, Entsch
B, Ballou DP, Chaiyen
P. LuxG is a functioning
flavin reductase for bacterial
luminescence. Journal
of Bacteriology 2008;190(5):1531-8. |
|
|
17. |
Sucharitakul
J, Prongjit M, Haltrich D, Chaiyen
P. Detection of a C4a-Hydroperoxyflavin
Intermediate in the Reaction of
a Flavoprotein Oxidase. Biochemistry 2008;47(33):8485–90. |
|
|
2552 |
18. |
Spadiut
O, Pisanelli I, Maischberger T,
Peterbauer C, Gorton L, Chaiyen
P, Haltrich D. Engineering
of pyyranose 2-oxidase: improvement
for biofuel cell and food applications
through semi-rational protein
design. Journal
of Biotechnology 2009;
139(3):250-7.
|
|
|
19. |
Prongjit M, Sucharitakul
J, Wongnate T, Haltrich D, Chaiyen
P. Kinetic mechanism
of pyranose 2-oxidase from Trametes
multicolor. Biochemistry 2009;48(19):4170-80. |
|
|
20. |
Chosrowjan H, Taniguchi
S, Mataga N, Phongsak T, Sucharitakul
J, Chaiyen P,
Tanaka F. Ultrafast Solvation
Dynamics of flavin mononucleotide
in the reductase component of
p-Hydroxyphenylacetate Hydroxylase. Journal
of Physical Chemistry B 2009;113(25):8439-42. |
|
|
21. |
Baron R, Riley
C, Chenprakhon P, Thotsaporn K,
Winter RT, Alfieri A, Forneris
F, van Berkel WJH, Chaiyen
P, Fraaije MW, Mattevi
A, McCammon JA. Multiple pathways
guide oxygen diffusion into flavoenzyme
active sites. Proceedings
of the National Academy of Sciences
of the United States of America
2009;106(25):10603-8. |
|
[ Cited in "Research Highlights"
Nature Chemistry, 2009] |
|
|
22. |
Sopitthammakhun
K, Maenpuen S, Yuthavong Y, Leartsakulpanich
U, Chaiyen P.
Serine hydroxymethyltransferase
from Plasmodium vivax is different
in substrate specificity from
its homologues. FEBS
Journal 2009;276(15):4023-36. |
|
|
23. |
Maenpuen S, Sopitthammakhun
K, Yuthavong Y, Chaiyen
P, Leartsakulpanich U.
Characterization of Plasmodium
falciparum serine hydroxymethyltransferase—A
potential antimalarial target. Molecular
and Biochemical Parasitology 2009;168(1):63-73. |
|
|
2553 |
24. |
Chaiyen
P. Flavoenzymes
catalyzing oxidative aromatic
ring-cleavage reactions. Arch
Biochem Biophys. 2010;493(1):62-70. (Review) |
|
|
25. |
Pitsawong W, Sucharitakul
J, Prongjit M, Tan TC, Spadiut
O, Haltrich D, Divne C, Chaiyen
P. A conserved active-site
threonine is important for both
sugar and flavin oxidations of
pyranose 2-oxidase. Journal
of Biological Chemistry 2010;285(13):9697-705. |
|
|
26. |
Sucharitakul J,
Wongnate T, Chaiyen P.
Kinetic isotope effects on the
noncovalent flavin mutant protein
of pyranose 2-oxidase reveal insights
into the flavin reduction mechanism. Biochemistry 2010;49(17):3753-65. |
|
|
27. |
Chenprakhon P,
Sucharitakul J, Panijpan B, Chaiyen P. Measuring
binding affinity of protein. Ligand
interaction using spectrophotometry:
binding of neutral red to riboflavin-binding
protein. Journal
Chemical Education 2010;87(8):829-31. |
|
|
28. |
Rujkorakarn R,
Nunthaboot N, Tanaka F, Chaiyen P, Chosrowjan
H, Taniguchi S, Mataga N. Time-resolved
Stokes shift in proteins with
continuum model: Slow dynamics
in proteins. Journal of Photochemistry and
Photobiology A: Chemistry 2010;215(1):38-45. |
|
|
29. |
Tan TC, Pitsawong
W, WongnateT, Spadiut O, Haltrich
D, Chaiyen P,
Divne C. H-bonding and Positive
Charge at the N(5)/O(4) Locus
are Critical for Covalent Flavin
Attachment in Trametes Pyranose
2-Oxidase. Journal of Molecular Biology 2010;402(3):578-94 |
|
|
30. |
Nijvipakul S, Ballou
SP, Chaiyen P.
Reduction Kinetics of a Flavin
Oxidoreductase LuxG from Photobacterium
leiognathi (TH1):Half Sites Reactivity. Biochemistry 2010;49 (43):9241–8 |
|
|
2554 |
31. |
Ruangchan N, Tongsook
C, Sucharitakul J, Chaiyen
P. pH-dependent studies
reveal an efficient hydroxylation
mechanism of the oxygenase component
of p-hydroxyphenylacetate 3-hydroxylase. Journal
of Biological Chemistry 2011;286(1):223-33. |
|
|
32. |
Sucharitakul J,
Wongnate T, Chaiyen P. Hydrogen peroxide elimination
from C4A-hydroperoxy-flavin in
a flavoprotein oxidase occurs
through a single proton transfer
from flavin N5 to a peroxide leaving
group. Journal
of Biological Chemistry 2011;286(19):16900-9. |
|
|
33. |
Thotsaporn K, Chenprakhon P, Sucharitakul J, Mattevi A, Chaiyen P. Stabilization of C4A-hydroperoxy-flavin in a two-component flavin-dependent monooxygenase is achieved through interactions at flavin N5 and C4a atoms. Journal
of Biological Chemistry 2011;286(32):28170-80. |
|
|
34. |
Penpassakarn P, Chaiyen P, Palittapongarnpim P. Efficient heterologous expression and one-step purification of fully active c-terminal histidine-tagged uridine monophosphate kinase from Mycobacterium tuberculosis. The Southeast Asian Journal of Tropical Medicine and Public Health 2011;42(6):1452-1459. |
|
|
35. |
Wongnate T, Sucharitakul J , and Chaiyen P. Identification of a catalytic base for sugar oxidation in pyranose 2-oxidase reaction. Chembiochem 2011;12(17):2577-86. |
|
|
36. |
Tongsook C, Sucharitakul J, Thotsaporn K, Chaiyen P. Interactions with the substrate phenolic group are essential for hydroxylation by the oxygenase component of p-hydroxyphenylacetate 3-hydroxylase. Journal of Biological Chemistry 2011;286(52):44491–502. |
|
|
2555 |
|
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37. |
Chenprakhon P, Panijpan B, Chaiyen P. An Experiment Illustrating the Change in Ligand pKa upon Protein Binding. Journal of Chemical Education 2012; 89:791−795. |
|
|
38. |
Chosrowjan H, Taniguchi S, Wongnate T, Sucharitakul J, Chaiyen P, Tanaka F. Conformational heterogeneity in pyranose 2-oxidase from Trametes multicolor revealed by ultrafast fluorescence dynamics. Journal of Photochemistry and Photobiology A: Chemistry 2012;234(April 15):44-48. |
|
|
39. |
Oonnanat W, Sucharitakul J, Chaiyen P, Yuaniyama J. Crystallization and preliminary X-ray analysis of the reductase component of p-hydroxyphenylacetate 3-hydroxylase from Acinetobacter baumannii. Acinetobacter baumannii. Acta Crystallographica Section; F68:720–723. |
|
|
40. |
Sucharitakul J, Wongnate T, Montersino S, van Berkel, WJH, Chaiyen P. Reduction Kinetics of 3-Hydroxybenzoate 6-Hydroxylase from Rhodococcus jostii RHA1. Biochemistry 2012; 51(21):4309-21. |
|
|
41. |
Somprasong N, Jittawuttipoka T, Duang-nkern J, Romsang, A, Chaiyen P, Schweizer H, Vattanaviboon P, Mongkolsuk S. Pseudomonas aeruginosa thiol peroxidase protects against hydrogen peroxide toxicity and displays atypical patterns of gene regulation. Journal of Bacteriology 2012; 194(15):3904-12. |
|
|
42. |
Sopitthummakhun K, Thongpanchang C, Vilaivan T, Yuthavong Y, Chaiyen P, Leartsakulpanich U. Plasmodium serine hydroxymethyltransferase as a potential anti-malarial target: inhibition studies using improved methods for enzyme production and assay, Malaria Journal 2012;11(1):194. |
|
|
43. |
Phongsak T, Sucharitakul J, Thotsaporn K, Oonanant W, Yuvaniyama J, Svasti J, Ballou DP, Chaiyen P.The C-terminal domain of 4-hydroxyphenylacetate 3-hydroxylase from Acinetobacter baumannii is an auto-inhibitory domain. J. Biol.Chem. 2012 ; 287(31):26213-26222. |
|
|
44. |
Taniguchi S, Chosrowjan H, Wongnate T, Sucharitakul J, Chaiyen P, Tanaka F.
Ultrafast Fluorescence Dynamics of Flavin Adenine Dinucleotide in Pyranose 2-Oxidases Variants and Their Complexes with Acetate: Conformational Heterogeneity with Different Dielectric Constants. Journal of Photochemistry and Photobiology A: Chemistry 2012; 245:33-42. |
|
|
45. |
Chaiyen P, Fraaije M, Mattevi A. The Enigmatic Reaction of Flavins with Oxygen. Trends in Biochemical Sciences 2012;37(9):373-80. |
|
|
46. |
Tinikul R, Thotsaporn K, Taveekarn V, Jitrapakdee S, Chaiyen P. The fusion Vibrio campbellii luciferase as a eukaryotic gene reporter. Journal of Biotechnology 162 (2012):pp 346–353. |
|
|
2556 |
47. |
Tan TC, Spadiut O, Wongnate T, Sucharitakul J, Krondorfer I, Sygmund C, Haltrich D, Chaiyen P, Peterbauer CK, Divne C*. The 1.6 Å crystal structure of pyranose dehydrogenase from Agaricus meleagris rationalizes substrate Specificity and Reveals a Flavin Intermediate. PLoS One 2013;8(1):e53567. |
|
|
48. |
Prongjit M, Sucharitakul J, Palfey BA, Chaiyen P*. Oxidation mode of pyranose 2-oxidase is controlled by pH. Biochemistry 2013 Feb 26;52(8):1437-45. |
|
|
49. |
Wongnate T, Chaiyen P*. The substrate oxidation mechanism of pyranose 2-oxidase and other related enzymes in the glucose-methanol-choline superfamily. FEBS J 2013 Jul;280(13):3009-27. (Review) |
|
|
50. |
Tinikul R, Pitsawong W, Sucharitakul J, Nijvipakul S, Ballou DP, Chaiyen P. The transfer of reduced FMN from LuxG oxidoreductase to luciferase occurs via free diffusion. Biochemistry. 2013 Oct 1;52(39):6834-6843. |
|
|
51. |
Sucharitakul J*, Tongsook C, Pakotiprapha D, van Berkel WJ, Chaiyen P. The Reaction Kinetics of 3-Hydroxybenzoate 6-Hydroxylase from Rhodoccocus jostii RHA1 Provide an Understanding of the para-Hydroxylation Enzyme Catalytic Cycle. J Biol Chem. 2013 Dec 6;288:35210-35221. |
|
|
2557 |
|
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52. |
Wongnate T, Surawatanawong P, Visitsatthawong S, Sucharitakul J, Scrutton NS, Chaiyen P. Proton-coupled Electron Transfer and Adduct Configuration are Important for C4a-Hydroperoxyflavin Formation and Stabilization in a Flavoenzyme. J. Am. Chem. Soc. 2014; 136 (1): pp 241–253. |
|
|
53. |
Chitnumsub P, Ittarat P, Jaruwat A, Noytanom K, Amornwatcharapong W, Pornthanakasem W, Chaiyen P, Yuthavong Y, Leartsakulpanich U. Structure of Plasmodium falciparum serine hydroxymethyltransferase reveals a novel redox switch that regulates its activities. Acta Crystallogr D Biol Crystallogr. 2014 Jun;70(Pt 6):1517-27. |
|
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54. |
Pinthong C, Maenpuen S, Amornwatcharapong W, Yuthavong Y, Leartsakulpanich U, Chaiyen P.Distinct Biochemical Properties of Human Serine Hydroxymethyltransferase Compared to the Plasmodium Enzyme: Implications for Selective Inhibition. FEBS J. 2014 Jun;281(11):2570-83. |
|
55. |
Sucharitakul J, Tinikul R, Chaiyen P. Mechanisms of Reduced Flavin Transfer in the Two-component flavin-dependent Monooxygenases. Arch Biochem Biophys. 2014 Aug;555-556:33-46 |
|
|
56. |
Srisook T, Vongsetskula T*, Sucharitakul J, Chaiyen P, Tangboriboonrat P. Immobilization of 3-hydroxybenzoate 6-hydroxylase onto functionalized electrospun polycaprolactone ultrafine fibers: a novel heterogeneous catalyst. Reactive and Functional Polymers, 82, 41-46. |
|
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57. |
Chenprakhon P , Trisrivirat D, Thotsaporn K , Sucharitakul J, and Chaiyen P* Control of C4a-hydroperoxyflavin protonation in the oxygenase component of p-hydroxyphenyl acetate-3-hydroxylase. Biochemistry 2014 Jul 1;53(25):4084-6. (Rapid Report) |
|
|
58. |
Chitnumsub, P., Jaruwat, A., Riangrungroj, P., Ittarat, W., Noytanom, K., Oonanant, W., Vanichthanankul, J., Chuankhayan, P., Maenpuen, S., Chen, C.-J., Chaiyen, P., Yuthavong, Y., Leartsakulpanich, U.
Structures of Plasmodium vivax serine hydroxymethyltransferase: Implications for ligand-binding specificity and functional control
(2014) Acta Crystallographica Section D: Biological Crystallography, 70 (12), pp. 3177-3186. |
|
|
59. |
Sahin, S., Wongnate, T., Chaiyen, P., Yu, E.H.
Glucose oxidation using oxygen resistant pyranose-2-oxidase for biofuel cell applications
(2014) Chemical Engineering Transactions, 41 (Special Issue), pp. 367-372. |
2558 |
|
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60. |
Messiha HL, Wongnate T, Chaiyen P, Jones AR, Scrutton NS. Magnetic field effects as a result of the radical pair mechanism are unlikely in redox enzymes. J R Soc Interface. 2015;12(103). |
|
|
61. |
Panatdasirisuk, W., Vongsetskul, T., Sucharitakul, J., Chaiyen, P., Tangboriboonrat, P. Functionalized electrospun regenerated cellulose fibers for immobilizing pyranose 2-oxidase. Reactive and Functional Polymers 2015;86:47-51. |
|
|
62. |
Luanloet T, Sucharitakul J and Chaiyen P*. Selectivity of Substrate Binding and Ionization of 2-Methyl-3-Hydroxypyridine-5-Carboxylic Acid Oxygenase. FEBS J. 2015; 282(16):3107-25. |
|
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63. |
Elkhal C.K., Kean K, Parsonage D, Maenpuen S, Chaiyen P, Claiborne A and Karplus P.A. Structure and proposed mechanism of alpha-glycerophosphate oxidase from Mycoplasma pneumonia. FEBS J. 2015; 282(16):3030-42. |
|
|
64. |
Maenpuen S, Amornwatcharapong W, Krasatong P, Sucharitakul J, Palfey BA, Yuthavong Y, Chitnumsub P, Leartsakulpanich U, Chaiyen P. Kinetic mechanism and the rate-limiting step of Plasmodium vivax serine hydroxymethyltransferase. Journal of Biological Chemistry 2015;290(13):8656-65. |
|
|
65. |
Maenpuen S, Watthaisong P, Supon P, Sucharitakul J, Parsonage D, Karplus PA, Claiborne A, Chaiyen P. Kinetic Mechanism of L-alpha-Glycerophosphate Oxidase from Mycoplasma pneumoniae. FEBS J. 2015; 282(16):3043-59. |
|
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66. |
Witschel MC, Rottmann M, Schwab A, Leartsakulpanich U, Chitnumsub P, Seet M, Tonazzi S, Schwertz G, Stelzer F, Mietzner T, McNamara C, Thater F, Freymond C, Jaruwat A, Pinthong C, Riangrungroj P, Oufir M, Hamburger M, Mäser P, Sanz-Alonso LM, Charman S, Wittlin S, Yuthavong Y, Chaiyen P, Diederich F. Inhibitors of plasmodial serine hydroxymethyltransferase (SHMT): Cocrystal structures of pyrazolopyrans with potent blood- and liver-stage activities. Journal of Medicinal Chemistry 2015;58(7):3117-30. |
|
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67. |
Dhammaraj T, Phintha A, Pinthong C, Medhanavyn D, Tinikul R, Chenprakhon P, Sucharitakul J, Vardhanabhuti N, Jiarpinitnun C, and Chaiyen P. p-Hydroxyphenylacetate 3-hydroxylase as a biocatalyst for the synthesis of trihydroxyphenolic acids. ACS Catalysis 2015; 5:4492–4502. |
|
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68. |
Visitsatthawong S, Chenprakhon P, Chaiyen P, and Surawatanawong P. Mechanism of oxygen activation in a flavin-dependent monooxygenase: A nearly barrierless C4a-hydroperoxyflavin formation via Proton-coupled electron transfer. Journal of the American Chemical Society 2015; 137(29):9363-74. |
|
|
69. |
Chaiyen P, Scrutton NS. Special Issue: Flavins and Flavoproteins: Introduction.
FEBS J. 2015 Aug;282(16):3001-2. |
|
|
70. |
Graf M, Sucharitakul J, Bren U, Chu B, Koellensberger G; Hann S, Furtmüller P, Obinger C, Peterbauer C, Oostenbrink C. Chaiyen P, Haltrich, D. Reaction of pyranose dehydrogenase from Agaricus meleagris with its carbohydrate substrates. FEBS J. 2015 Nov;282(21):4218-41. |
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2559 |
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71. |
Sucharitakul J, Medhanavyn D, Pakotiprapha D, van Berkel W. Chaiyen P. Tyr217 and His213 are Important for Substrate Binding and Hydroxylation of 3-Hydroxybenzoate 6-Hydroxylase from Rhodococcus jostii. FEBS J 2016 Mar;283(5):860-81. |
|
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72. |
Tinikul R and Chaiyen P.
Structure, Mechanism, and Mutation of Bacterial Luciferase In: Advances in Biochemical Engineering/Technology (Springer-Verlag Berlin Heidelberg) 2016; 154:47-74. |
|
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73. |
Dhammaraj T, Pinthong C, Visitsatthawong S, Tongsook C, Surawatanawong P, Chaiyen P. A Single-site Mutation at Ser146 Expands the Reactivity of the Oxygenase Component of p-Hydroxyphenylacetate 3-Hydroxylase. ACS Chem Biol. 2016;11(10):2889-2896. |
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74. |
Thotsaporn K, Tinikul R, Maenpuen S, Phonbuppha J, Watthaisong P, Chenprakhon P, and Chaiyen P. Enzymes in the p-hydroxyphenylacetate degradation pathway of Acinetobacter baumannii. Journal of Molecular Catalysis B: Enzymatic 134 (2016) 353–366. |
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2560 |
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75. |
Pruksatrakul T, PPhoopraintra P, Wilairat P, Chaiyen P, Chantiwas R. Development of a sequential injection-liquid microextraction procedure with GC-FID for analysis of short-chain fatty acids in palm oil mill effluent. Talanta 2017; 165 (1): 612-618. |
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76. |
Pornsuwan S, Maenpuen S, Kamutira P, Watthaisong P, Thotsaporn K, Tongsook C, Juttulapa M, Nijvipakul S, Chaiyen P. 3,4-Dihydroxyphenylacetate 2,3-dioxygenase from Pseudomonas aeruginosa: An Fe(II)-containing enzyme with fast turnover. PLOS ONE 2017; February (3): 1-25. |
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77. |
Pimviriyakul P, Thotsaporn K, Sucharitakul J and Chaiyen P. Kinetic Mechanism of the Dechlorinating Flavin-Dependent Monooxygenase HadA. J Biol Chem. 2017 Mar 24;292(12):4818-4832 |
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78. |
Chenprakhon P, Dhammaraj T, Chantiwas R, Chaiyen P. Hydroxylation of 4-hydroxyphenylethylamine derivatives by R263 variants of the oxygenase component of p-hydroxyphenylacetate-3-hydroxylase. Arch Biochem Biophys. 2017 Apr 15;620:1-11. |
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79. |
Schwertz G, Witschel MC, Rottmann M, Bonnert R, Leartsakulpanich U, Chitnumsub P, Jaruwat A, Ittarat W, Schäfer A, Aponte RA, Charman SA, White KL, Kundu A, Sadhukhan S, Lloyd M, Freiberg GM, Srikumaran M, Siggel M, Zwyssig A, Chaiyen P, Diederich F. Antimalarial Inhibitors Targeting Serine Hydroxymethyltransferase (SHMT) with in Vivo Efficacy and Analysis of their Binding Mode Based on X-ray Cocrystal Structures.
J Med Chem. 2017 Jun 22;60(12):4840-4860. |
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80. |
Amornwatcharapong W, Maenpuen S, Chitnumsub P, Leartsakulpanich P, and Chaiyen P. Human and Plasmodium Serine Hydroxymethyl Transferases Differ in Rate-limiting Steps and pH-Dependent Substrate Inhibition Behavior. Archives of Biochemistry and Biophysics 630 (2017), 91-100. |
|
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81. |
Pinthong C , Phoopraintra P, Chantiwas R, Pongtharangkul T, Chenprakhon P, and Chaiyen P. Green and Sustainable Biocatalytic Production of 3,4,5-Trihydroxycinnamic Acid from Palm Oil Mill Effluent. Process Biochemistry. 2017; 63: 122-129. |
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82. |
Schwertz G, Frei MS, Witschel MC, Rottmann M, Leartsakulpanich U, Chitnumsub P, Jaruwat A, Ittarat A, Schäfer A, Aponte RA, Trapp N, Mark K, Chaiyen P, Diederich F. Conformational Aspects in the Design of Inhibitors for Serine Hydroxymethyltransferase (SHMT): Biphenyl, Aryl Sulfonamide, and Aryl Sulfone Motifs, Chemistry - A European Journal. 2017; 23(57): 14345-14357. |
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2561 |
83. |
Sawasdee K, Sucharitakul J, Dhammaraj T, Niamsiri N,
Chaiyen P, Prapainop K
Encapsulation of the reductase component of
p-Hydroxyphenylacetate Hydroxylase in poly
(lactide-co-glycolide) nanoparticles by three different
emulsification techniques. IET
Nanobiotechnology. 2018 Jun;12(4):423-428. .
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84. |
Tinikul R, Chenprakhon P, Maenpuen S, Chaiyen
P. Biotransformation of Plant-Derived Phenolic
Acids. Phenolic
Acids. Biotechnol J. 2018 Jun;13(6):e1700632.
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85. |
şahin S, Wongnate T, Chuaboon L, Chaiyen
P, Yu EH. Enzymatic fuel cells with an oxygen
resistant variant of pyranose-2-oxidase as anode biocatalyst.
Biosens
Bioelectron. 2018. pp 17-25.
|
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86. |
Teanphonkrang S, Janke S, Chaiyen P,
Sucharitakul J, Suginta W, Khunkaewla P, Schuhmann W, Ruff A,
Schulte A. Tuned Amperometric Detection of Reduced
β-Nicotinamide Adenine Dinucleotide by Allosteric
Modulation of the Reductase Component of the
p-Hydroxyphenylacetate Hydroxylase Immobilized within a Redox
Polymer.Analytical
Chemistry, Anal. Chem., 2018, 90 (9), 5703-5711. .
|
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87. |
Schwertz G, Witschel MC, Rottmann M,
Leartsakulpanich U, Chitnumsub P, Jaruwat A, Amornwatcharapong
W, Ittarat W, Schäfer A, Aponte RA, Chaiyen
P, and Diederich F. Potent Inhibitors for Plasmodial
Serine Hydroxymethyltransferase (SHMT) Featuring a Spirocyclic
Scaffold, ChemMedChem.
2018 May 8;13(9):931-943. |
|
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88. |
Phonbuppha J, Maenpuen S, Munkajohnpong P,
Chaiyen P, Tinikul R. A selective determination
of the catalytic cysteine pKa of 2-cysteine succinic
semialdehyde dehydrogenase from Acinetobacter baumannii using
burst kinetics and enzyme adduct formation, FEBS J. 2018
Jul;285(13):2504-2519. |
|
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89. |
Yuenyaw A, Petchyam N, Kamonsutthipaijit N, Chaiyen
P, Pakotiprapha D. Crystal structure of the flavin
reductase of Acinetobacter baumannii p-hydroxyphenylacetate
3-hydroxylase (HPAH) and identification of amino acid residues
underlying its regulation by aromatic ligands. Archives of
Biochemistry and Biophysics. 2018 Sep 1; 653:24-38.
|
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90. |
Pimviriyakul P, Surawatanawong P, Chaiyen P.
Oxidative Dehalogenation and Denitration by a Flavin-dependent
Monooxygenase is Controlled by Substrate Deprotonation. Chemical
Science. 2018 (9); 7468 – 7482.
|
|
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91. |
Pimviriyakul P, Chaiyen P. A complete
bioconversion cascade for dehalogenation and denitration by
bacterial flavin-dependent enzymes. J Biol
Chem. 2018 Nov 30;293(48):18525-18539.
|
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2562 |
92. |
Chenprakhon P, Wongnate T, Chaiyen P.
Monooxygenation of aromatic compounds by flavin-dependent
monooxygenases. Protein Sci. 2019
Jan;28(1):8-29.
|
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93. |
Chuaboon L, Wongnate T, Punthong P, Kiattisewee C, Lawan N,
Hsu CY, Lin CH, Bornscheuer U, Chaiyen P.
One-Pot Bioconversion of L-Arabinose to L-Ribulose in an
Enzymatic Cascade. Angew Chem Int
Ed Engl. 2019 Feb 18;58(8):2428-2432.
|
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94. |
Wongnate T, Surawatanawong P, Chuaboon L, Lawan N,
Chaiyen P. The mechanism of sugar C-H bond
oxidation by a flavoprotein oxidase occurs by a hydride
transfer before proton abstraction. Chemistry.
2019 Mar 21;25(17):4460-4471.
|
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95. |
Teanphonkrang S, Ernst A, Janke S, Chaiyen
P, Sucharitakul J, Suginta W, Khunkaewla P,
Schuhmann W, Schulte A, Ruff A. Amperometric detection of the
urinary disease biomarker p-HPA by allosteric modulation of a
redox polymer-embedded bacterial reductase. ACS Sens.
2019 May 24;4(5):1270-1278.
|
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96. |
Duangpummet P, Chaiyen P, Chenprakhon P.
Lipase-Catalyzed Esterification: An Inquiry-Based Laboratory
Activity to Promote High School Students' Understanding and
Positive Perceptions of Green Chemistry. J Chem
Educ. 2019 May;96(6):1205-1211.
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97. |
Ubonprasert S, Jaroensuk J, Pornthanakasem W,
Kamonsutthipaijit N, Wongpituk P, Mee-Udorn P, Rungrotmongkol
T, Ketchart O, Chitnumsub P, Leartsakulpanich U,
Chaiyen P, Maenpuen S. A flap motif in human
serine hydroxymethyl transferase is important for structural
stabilization, ligand binding, and control of product release.
J Biol
Chem. 2019 Jul 5;294(27):10490-10502.
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98. |
Jaroensuk J, Intasian P, Kiattisewee C, Munkajohnpon P,
Chunthaboon P, Buttranon S, Trisrivirat D, Wongnate T,
Maenpuen S, Tinikul R, Chaiyen P. Addition of
formate dehydrogenase increases the production of renewable
alkane from an engineered metabolic pathway. J Biol Chem. 2019 Jul 26;294(30):11536-11548.
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|
99. |
Pongpamorn P, Watthaisong P, Pimviriyakul P, Jaruwat A, Lawan N, Chitnumsub P, Chaiyen P. Identification of a Hotspot Residue for Improving the Thermostability of a Flavin-Dependent Monooxygenase. Chembiochem. 2019 Dec 13;20(24):3020-3031.
|
|
|
100. |
Watthaisong P, Pongpamorn P, Pimviriyakul P, Maenpuen S,
Ohmiya Y, Chaiyen P. A Chemo-Enzymatic
Cascade for the Smart Detection of Nitro- and Halogenated
Phenols. Angew Chem Int Ed Engl. 2019 Sep;58(38):13254-13258
- Highlighted as "Hot
Paper:Biocatalysis" and "Frontispiece Article"
- Highlighted
in more than 8 news outlets
|
101. |
Jaroensuk J, Wong YH, Zhong W, Liew CW, Maenpuen S, Sahili AE, Atichartpongkul S, Chionh YH, Nah Q, Thongdee N, McBee ME, Prestwich EG, DeMott MS, Chaiyen P, Mongkolsuk S, Dedon P, Lescar J, Fuangthong M. Crystal structure and catalytic mechanism of the essential m1G37 tRNA methyltransferase TrmD from Pseudomonas aeruginosa. RNA. 2019 Nov;25(11):1481-1496.
|
|
|
102. |
Thongdee N, Jaroensuk J, Atichartpongkul S, Chittrakanwong J, Chooyoung K, Srimahaeak T, Chaiyen P, Vattanaviboon P, Mongkolsuk S, Fuangthong M. TrmB, a tRNA m7G46 methyltransferase, plays a role in hydrogen peroxide resistance and positively modulates the translation of katA and katB mRNAs in Pseudomonas aeruginosa. Nucleic Acids Res. 2019 Sep;47(17):9271-9281.
|
|
|
103. |
Songsungthong W, Yongkiettrakul S, Bohan LE, Nicholson ES, Prasopporn S, Chaiyen P, Leartsakulpanich U. Diaminoquinazoline MMV675968 from Pathogen Box inhibits Acinetobacter baumannii growth through targeting of dihydrofolate reductase. Sci Rep. 2019 Oct;9(1):15625.
|
|
|
2563 |
104. |
Pimviriyakul P, Wongnate T, Tinikul R, Chaiyen P. Microbial degradation of halogenated aromatics: molecular mechanisms and enzymatic reactions. Microb Biotechnol. 2020;13(1):67–86.
|
|
|
105. |
Jaroensuk J, Intasian P, Wattanasuepsin W, Akeratchatapan N, Kesornpun C, Kittipanukul N, Chaiyen P. Enzymatic reactions and pathway engineering for the production of renewable hydrocarbons. J Biotechnol. 2020 Feb 10;309:1-19.
|
|
|
106. |
Phonbuppha J, Tinikul R, Wongnate T, Intasian P, Hollmann F, Paul CE, Chaiyen P. A Minimized Chemoenzymatic Cascade for Bacterial Luciferase in Bioreporter Applications. Chembiochem, inpress.
|
|
|
107. |
Pitsawong W, Chenprakhon P, Dhammaraj T, Medhanavyn D, Sucharitakul J, Tongsook C, van Berkel WJH, Chaiyen P, Miller AF. Tuning of pKa values activates substrates in flavin-dependent aromatic hydroxylases. J Biol Chem. 2020 Mar 20;295(12):3965-3981.
|
|
|
108. |
Maenpuen S, Pongsupasa V, Pensook W, Anuwan P, Kraivisitkul N, Pinthong C, Phonbuppha J, Luanloet T, Wijma HJ, Fraaije MW, Lawan N, Chaiyen P, Wongnate T. Rational-design engineering to create the flavin reductase variants with thermostable and solvent-tolerant properties. Chembiochem, inpress.
|
|
|
109. |
Sucharitakul J, Buttranon S, Wongnate T, Chowdhury NP, Prongjit M, Buckel W, Chaiyen P. Modulations of the reduction potentials of flavin-based electron bifurcation complexes and semiquinone stabilities are key to control directional electron flow. FEBS J, inpress.
|
|
|
110. |
Munkajohnpong P, Kesornpun C, Buttranon S, Jaroensuk J, Weeranoppanant N, Chaiyen P. Fatty Alcohol Production: An Opportunity of Bioprocess. Biofuel Bioproduct Biorefinery, inpress.
|
|
|
111. |
Woraruthai T, Kunno J, Pongsopon M, Yansakon K, Phoopraintra P, Chantiwas R, Leartsakulpanich U, Chaiyen P, Wongnate T. Chaiyen P. Identification and Cultivation of Hydrogenotrophic Methanogens from Palm Oil Mill Effluent for High Methane Production. International Journal of Energy Research 2020, inpress
|
|
|
112. |
Trisrivirat D, Lawan N, Chenprakhon P, Matsui D, Asano Y, Chaiyen P. Mechanistic insights into the dual activities of the single active site of L-lysine oxidase/monooxygenase from Pseudomonas sp. AIU 813. J Biol Chem. 2020, inpress
|
|
|
บทความในหนังสือ
: |
1. |
Chaiyen P,
Suadee C, Meevoothisom V. Degradation
of phenylacetic acid by bacteria
from Thai soil. In: Annual Report
of ICBiotech. Vol. 21. Osaka:
International Center for Biotechnology;
1998. p. 862-7. |
|
|
2. |
Wongnate T and Chaiyen, P. Pyranose oxidases. In: Hille R., Miller, S.M., Palfey, B. (Eds.). Handbook of Flavoproteins. Oxidases, dehydrogenases and related systems. Volume 1. 2013. p. 177-193. |
|
|
3. |
Tinikul R and Chaiyen P. Structure, Mechanism, and Mutation of Bacterial Luciferase In: Advances in Biochemical Engineering/Technology (Springer-Verlag Berlin Heidelberg) 2016; 154:47-74. |
|
|
4. |
Maenpuen S, Tinikul S, Chenprakhon P and Chaiyen P. Production of Valuable Phenolic Compounds from Lignin by Biocatalysis: State-of-the-Art Perspective. In Emerging Areas in Bioengineering (2018), Wiley-VCH Verlag GmbH & Co. pp 102-124. |
|
บทความในหนังสือ
(รายงานการประชุมวิชาการ) : |
1. |
Chaiyen P,
Suadee C, Thotsaporn K, Svasti
J. Studies
of the two-component p-hydroxyphenylacetate
hydroxylase from Acinetobacter
baumannii. In: Chapman
S, Perham R, Scrutton N, editors.
Flavins and Flavoproteins 2002:
Proceedings of the Fourteenth
International Symposium; 2002
July 14-18; St. John's College,
University of Cambridge, Uk.
Berlin: Agency for Scientific
Publications; 2002. p. 975-80.
|
2. |
Sucharitakul J, Chaiyen
P, Ballou DP, Massey
V. Probing
the mechanism of 2-methyl-3-hydroxypyridine-5-carboxylic
acid oxygenase by using 8-substituted-FAD
analogs. In: Chapman S,
Perham R, Scrutton N, editors.
Flavins and Flavoproteins 2002:
Proceedings of the Fourteenth
International Symposium; 2002
July 14-18; St. John's College,
University of Cambridge, Uk.
Berlin: Agency for Scientific
Publications; 2002. p. 381-6.
|
3. |
Sucharitakul J, Chaiyen
P, Entsch B, Ballou
DP. Mechanistic
studies on the reductase component
of p-hydroxyphenylacetate
3-hydroxylase from Acinetobacter
baumannii.. In: Nishino
T, Miura R, Tanokura M, Fukui
K, editors. Flavins and Flavoproteins
2005: Proceedings of the Fifteenth
International Symposium; 2005
April 17-22; Shonan Village
Center, Hayama, Japan: ARchiTect
Inc.; 2005. p. 275-80.
|
4. |
Chaiyen P,
Phongsak T, Sucharitakul J,
Ballou DP. Review
and perspectives of how reduced
flavin is transferred between
the reductase and oxygenase
p-hydroxyphenylacetate 3-hydroxylase.
In: Frago S, Gomez-moreno C,
Medina M, editors. Flavins and
Flavoproteins 2008: Proceedings
of the Sixteenth International
Symposium; 2008 June 8-13; Jaca,
Spain: Prensas Universitarias
de Zaragoza; 2008. p. 27-44. |
Patent: |
2555 |
ยีนสำหรับฟิวชั่นลูซิเฟอเรสเอนไซม์จากเชื้อแบคทีเรีย Vibrio
campbellii
ที่เสถียรต่อภาวะอุณหภูมิสูงและองค์ประกอบของน้ำยาสำหรับวัดแอคติวิตี้เอนไซม์ลูซิเฟอเรส
|
สิทธิบัตร
(1201000720) |
2559 |
Fusion bacterial luciferase gene, reporter vector, and assay kit |
Patent Cooperation Treaty, PCT (PCT/TH/2016000051) |
2561 |
องค์ประกอบเครื่องหมายโปรตีน (Protein Marker) |
อนุสิทธิบัตร (เลขที่คำขอ 1503001543 เลขที่อนุสิทธิบัตร 14519) |
|
องค์ประกอบเครื่องหมายโปรตีนที่ติดสี (Pre-stained Protein
Marker) |
อนุสิทธิบัตร (เลขที่คำขอ 1503001542 เลขที่อนุสิทธิบัตร 13888) |
|
บทความในการประชุมวิชาการ (ระดับนานาชาติ)
|
1.
|
Chaiyen P,
Suadee C, Thotsaporn K, Svasti
J. Studies
of the two-component p-hydroxyphenylacetate
hydroxylase from Acinetobacter
baumannii. In: Chapman
S, Perham R, Scrutton N, editors.
Flavins and Flavoproteins 2002:
Proceedings of the Fourteenth
International Symposium; 2002
July 14-18; St. John's College,
University of Cambridge, Uk.
Berlin: Agency for Scientific
Publications; 2002. p. 975-80.
|
2.
|
Sucharitakul J, Chaiyen
P, Ballou DP, Massey
V. Probing
the mechanism of 2-methyl-3-hydroxypyridine-5-carboxylic
acid oxygenase by using 8-substituted-FAD
analogs. In: Chapman S,
Perham R, Scrutton N, editors.
Flavins and Flavoproteins 2002:
Proceedings of the Fourteenth
International Symposium; 2002
July 14-18; St. John's College,
University of Cambridge, Uk.
Berlin: Agency for Scientific
Publications; 2002. p. 381-6.
|
3.
|
Sucharitakul J, Chaiyen
P, Entsch B, Ballou
DP. Mechanistic
studies on the reductase component
of p-hydroxyphenylacetate
3-hydroxylase from Acinetobacter
baumannii.. In: Nishino
T, Miura R, Tanokura M, Fukui
K, editors. Flavins and Flavoproteins
2005: Proceedings of the Fifteenth
International Symposium; 2005
April 17-22; Shonan Village
Center, Hayama, Japan: ARchiTect
Inc.; 2005. p. 275-80.
|
4.
|
Chaiyen P,
Phongsak T, Sucharitakul J,
Ballou DP. Review
and perspectives of how reduced
flavin is transferred between
the reductase and oxygenase
p-hydroxyphenylacetate 3-hydroxylase.
In: Frago S, Gomez-moreno C,
Medina M, editors. Flavins and
Flavoproteins 2008: Proceedings
of the Sixteenth International
Symposium; 2008 June 8-13; Jaca,
Spain: Prensas Universitarias
de Zaragoza; 2008. p. 27-44.
|
|
บรรยายในการประชุมวิชาการ
(ระดับนานาชาติ) : |
1.
|
Oral Presentation
"p-Hydroxyphenylacetic
acid hydroxylase from Acinetobacter
baumannii" 15th the Federation
of Asian and Oceanian Biochemists
and Molecular Biologists (FAOBMB)
Symposium, Beijing, China, October
21-24, 2001. |
|
|
2.
|
Invited Seminar Lecture
"Reduced Flavin Transfer
between Components of p-Hydroxyphenyl-
acetate 3-Hydroxylase". A
seminar at University of Pavia,
Italy, June 12, 2006. |
|
|
3.
|
Invited Symposium Lecture
"How the reductase and oxygenase
work together in a two-component
flavoenzyme, p-hydroxyphenylacetate".
16th
International Symposium on Flavins
and Flavoproteins, Jaca, Spain
, Jun 10, 2008. |
|
|
4. |
Invited
Speaker
Gordon Research Conference 2010:
Enzymes, Co-enzymes & Metabolic
Pathways "What is necessary
for C4a-hydroperoxy-FAD formation
in pyranose 2-oxidase reacion?"
July 19-23, 2010 at Waterville
Valley Resort, New Hampshire,
USA. |
|
|
5. |
Invited seminar lecture
" Exploring the Reaction
Mechanism of Pyranose 2-Oxidase
Through Transient Kinetics and
Site-Directed Mutagenesis"
at Wake Forest University, July
27, 2010, Winston-Salem, North
Carolina, USA. |
|
|
6. |
Invited Symposium lecture
“Oxygenation mechanism of p-hydroxyphenylacetate
hydroxylase, a two-component flavin-dependent
monooxygenase”. The 3rd Asia Pacific Protein Association
(APPA) Conference, May 6-9, Shanghai, China. |
|
|
7. |
Invited Symposium lecture
“Use of Kinetic Isotope Effects and Transient Kinetics to
Unravel the Mechanism of H2O2 Elimination from
C4a-Hydroperoxy-flavin in Pyranose2-Oxidase”. The IXth
European Symposium of The Protein Society. May 22-26, 2011,
Stockholm, Sweden. |
|
|
8. |
Invited Symposium lecture
“Understanding reaction mechanisms of two-component
flavin-dependent monooxygenases through a model of
p-hydroxyphenylacetate hydroxylase”. 17th International
Conference on Cytochrome P450, at The University of Manchester,
UK 26th to 30th of June, 2011. |
|
|
9. |
Oral Presentation
Mechanistic Studies of Flavin- and PLP-Dependent Enzymes.
Presentation by TWAS young affiliate at 22nd General Meeting at
International centre in Theroretical Physics, Trieste, Italy, on
21-23 November 2011. |
|
|
10. |
Invited Speaker
Gordon Research Conference 2012: Biocatalysis "
Biocatalysis by Flavoenzymes:From Enzyme Mechanisms to
Rational Engineering”
July 8-13, 2012, Bryant University, Smithfield, RI, USA.
|
|
|
11. |
Invited Symposium Lecture
"Control and Versatility in Catalysis by Flavin-dependent
Enzymes"
Enzyme Engineering XXII: Emerging Topics in Enzyme
Engineering
September 22-26, 2013, Toyama International Conference Center,
Toyama, Japan
|
|
|
12. |
Invited Lecture
"Bacterial Luciferase from Thailand as a Gene
Reporter"
at BioMedical Research Institute, AIST, Tsukuba, Ibaraki,
Japan
|
|
|
13. |
Invited Lecture
"Aromatic flavin-dependent monooxygenase as a
regio-specific biocatalyst"
May 12, 2014 at Institute of Biochemistry, University of
Greifswald, Greifswald, Mecklenburg-Vorpommern,
Germany.
|
|
|
14. |
Invited Lecture
“Oxygen Activation by Flavin-Dependent Enzymes”
May 14, 2014 at The Max Planck Institute (MPI) for Terrestrial
Microbiology, Marburg, Germany.
|
|
|
15. |
Invited Lecture
Invited Lecture “Mechanistic Studies of Flavin- and
PLP-dependent Enzymes”
at BASF-The Chemical Company, Ludwigshafen,
Germany.
|
|
|
16. |
Invited Lecture
"Mechanisms of Oxygen Activation by Flavin-Dependent Enzymes"
October 22, 2014 at 15th IUBMB - 24th
FAOBMB-TSBMB International Conference, Academia Sinica,
Taipei, Taiwan
|
|
|
17. |
Invited Lecture
"Mechanism and Biocatalysis of Flavin-Dependent
Oxygenases"
13 November, 2014 at 9th Joint Conference on
Chemistry Program
Semarang, Indonesia.
|
|
|
18. |
Invited Lecture
Gordon Research Conference 2015: Enzymes, Co-enzymes &
Metabolic Pathways "Oxygenation and Beyond by
Two-component Flavin-dependent monooxygenases" July
12-17, 2015 at Waterville Valley Resort, New Hampshire, USA.
|
|
|
19. |
Invited Lecture
“Biotransformation of Aromatic Compounds by
Flavin-Dependent Monooxygenases” July 26 – 30,
2015 at the 12th Biotrans, Vienna, Austria
|
|
|
20. |
Invited Lecture
"Two-component Flavin-Dependent Monooxygenases:
From Aromatic Hydroxylation to Light-Emitting Reaction"
September 16, 2015
at BioMedical Research Institute, AIST, Tsukuba, Ibaraki,
Japan
|
|
|
21. |
Invited Lecture
“From Mechanisms to Application of
Two-component Flavin-Dependent Monooxygenases” Sep
19-20, 2015 at a regional meeting of Japan Society for
Bioscience, Biotechnology and Agrochemistry, Toyama
Prefectural University, Toyama, Japan
|
|
|
22. |
Invited Lecture
"Two-component
Flavin-Dependent Monooxygenases: Challenge and Opportunity"
Oct 9, 2015 at BallouFest Symposium 2015 in honor of Professor
Dr. David P. Ballou, Department of Biological Chemistry,
University of Michigan, Ann Arbor, MI, USA.
|
|
|
23. |
Invited Lecture
"Bacterial Luciferase as a Eukaryotic Reporter
System" at 19th International Symposium on Bioluminescence and
Chemiluminescence, May 29 - June 2, Tsukuba, Japan.
|
|
|
24. |
Invited Lecture
(Plenary Lecture) "Oxygenation and Beyond by
Two-Component Flavin-Dependent Monooxygenases"
21th BEST Conference June 24-25, 2016, National Central
University, Taoyuan, Taiwan.
|
|
|
25. |
Invited Lecture
"From Mechanistic Understanding to Applications of
Two-Component
Flavin-Dependent Monooxygenases" at 8th European Meeting on
OxiZymes, OxiZymes 2016, 3-6 July, 2016, The Netherlands.
|
|
|
26. |
Invited Lecture
(Plenary Lecture) " Flavin-Dependent
Monooxygenases:One Intermediate for Many Reactions " The Fifth
International Conference on Cofactors (ICC-05) and Active
Enzyme Molecule 2016 (AEM 2016)” September 4 to 8, 2016,
Unazuki, Toyama, Japan.
|
|
|
27. |
Invited Lecture
"Dehalogenase and Pyranose 2-oxidases and their
applications" September 9, 2016 at R&D Headquarter, Amano
Enzyme, Gifu, Japan.
|
|
|
28. |
Invited Lecture
“Flavin-Dependent Monooxygenases for
Sustainable Technology” University of
Bristol, Department of Chemistry, UK, March 2017
|
|
|
29. |
Invited Lecture
“Flavin-Dependent Monooxygenases for
Sustainable Technology” University of Manchester,
Manchester Institute of Biotechnology, UK, March 2017
|
|
|
30. |
Invited Lecture
“Flavin-Dependent Monooxygenases for
Sustainable Technology” Laboratory of Molecular Biology
(LMB), Cambridge, UK, March 2017
|
|
|
31. |
Invited Lecture
(Plenary Lecture) "Insights into Mechanisms of
Flavin-Dependent Monooxygenases
and Beyond " 19th International Symposium on Flavins and
Flavoproteins,
Groningen, The Netherlands, July 2-6, 2017
|
|
|
32. |
Invited Lecture
"Beyond Monooxygenation by Flavin-Dependent
Enzymes " 20th International Conference on Cytochrome
P450: Biochemistry, Biophysics and Biotechnology
Düsseldorf, 27-31 August 2017
|
|
|
33. |
Invited Symposium Lecture
" From Fundamental to Novel Detection Technology of
Flavin-Dependent Monooxygenases" 2018 Protein Science Society
of Japan Annual Meeting, 26-28 June 2018, Niigata, Japan.
|
|
|
34. |
Invited Lecture
"From Fundamental Enzyme Catalysis To Applications in
Detection and Green Chemistry" at Institute for Integrated
Cell-Material Sciences (iCeMS), 29 June 2018, Kyoto, Japan
|
|
|
35. |
Invited Symposium Lecture
"Novel Enzymatic and Cascade Reactions for
Biocatalysis and Biodetection"at EMBO workshop: Enzymes,
biocatalysis and chemical biology: The new frontiers, 09 - 12
September 2018 Pavia, Italy, University of Pavia, Italy 9-12
September 2018
|
|
|
36. |
Invited Symposium Lecture
"Flavin-dependent dehalogease and halogenase"at 25
Year Biochemistry Workshopt at Technical University Dresden,
Dresden, Germany, 28 September 2018
|
|
|
37. |
Invited Lecture
"Versatile Reactions of Two-Component Flavin-Dependent
Monooxygenases" at Department of Experimental Biology &
RECETOX, Faculty of Science, Masaryk University, Brno, Czech
Republic, 1 November 2018
|
|
|
38. |
Invited Lecture
"Versatile Reactions of Two-Component Flavin-Dependent
Monooxygenases" at Department of Biochemistry, Wageningen
University, Wageningen, The Netherlands, 2 November 2018
|
|
|
39. |
Invited Lecture
"Flavin-Dependent Enzymes: Mechanisms and Innovations" at
Department of Chemistry, University of California, Berkeley,
CA, USA 23 April 2019.
|
|
|
40. |
Distinguished Alumni Lecture
"Flavin-Dependent Enzymes: Mechanisms and Innovations" at
Department of Biological Chemistry, University of Michigan,
Ann Arbor, USA 25 April 2019.
|
|
|
41. |
Keynote Lecture
"Novel Enzymatic Cascades for Biodetection, Biocatalysis and
Biofuel" at Southeast Asia Catalysis Conference (SACC),
National University of Singapore, Singapore, 23-24 May, 2019
|
|
|
42. |
Invited Lecture
"Enzymatic Cascades for Biocatalysis, Biodetection and
Biofuel" at Biotrans 2019, Groningen, The Netherlands. July
7-11, 2019.
|
|
|
43. |
Invited Lecture
27th FAOBMB & 44th MSBMB Conference and IUBMB Special
Symposia, Berjaya Times Square Hotel, Kuala Lumpur, Malaysia.
19-22 August 2019.
|
|
|
44. |
Invited Lecture
"Microbial enzymatic systems for converting agro-industrial
waste to useful compounds" at International Symposium on the
Genetics of Industrial Microorganisms (GIM 2019), Pisa, Italy,
8-11 September 2019.
|
|
|
45. |
Invited Lecture
"Biodetoxification, Biosynthesis and Bioluminescence from
Phenolic Toxicants: Three applications in a One-Pot Reaction"
at An Annual Symposium of Japan Association for
Bioluminescence and Chemiluminescence (JABC). Tsukuba, Japan.
5 October 2019.
|
|
|
46. |
Invited Lecture
at Multi-step Enzyme Catalyzed Processes Conference
(MECP2020), Aachen, Germany. 30 March – 2 April, 2020.
|
|
|
47. |
Invited Lecture
at the World Conference on Protein Science, Sapporo, Japan.
7-10 July, 2020.
|
|
|
|
บรรยายในการประชุมวิชาการ
(ระดับชาติ) : |
|
มากกว่า 80 ครั้ง |
|
|
|
|